Expression and function of BMP3 during chick limb development
Expression and function of BMP3 during chick limb development
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DOI：
10.1002/dvdy.21561
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发表时间：
2008-06-01
影响因子：
2.5
通讯作者：
Rosen, Vicki
中科院分区：
文献类型：
--
作者：
Gamer, Laura W.;Ho, Victoria;Rosen, Vicki
骨形态发生蛋白（BMP）在骨骼发育和骨稳态的许多方面发挥着不同的作用。在软骨内骨化过程中，需要BMP活性的严格调节以确保骨骼祖细胞适当存活、增殖和分化成软骨细胞和成骨细胞。BMP 3是BMP家族的一个结构上不同的成员，通过限制BMP信号转导而作为骨形成的负调节剂。在这项研究中，我们专注于鸡肢，我们发现BMP 3具有独特的定位模式，在发育中的软骨膜中有强表达。在软骨形成开始时，使用可复制逆转录病毒在鸡翅芽中过表达BMP 3，减少BMP信号传导，导致细胞增殖增加和细胞分化延迟，导致骨骼元件扩张和关节融合。我们的研究结果表明，BMP 3在软骨膜中的表达可能有助于调节软骨细胞增殖，通过调节BMP信号的水平，从而确保适当的软骨内骨化。
Bone morphogenetic proteins (BMPs) play diverse roles in many aspects of skeletal development and bone homeostasis. During endochondral ossification, tight regulation of BMP activity is required to assure proper survival, proliferation and differentiation of skeletal progenitor cells into chondrocytes and osteoblasts. BMP3, a structurally divergent member of the BMP family, acts as a negative regulator of bone formation by limiting BMP signal transduction. In this study, we focus on the chick limb where we find BMP3 has a unique localization pattern with strong expression in the developing perichondrium. Overexpression of BMP3 in chick wing bud at the onset of chondrogenesis, using replication competent retrovirus, reduces BMP signaling leading to increased cell proliferation and delayed cell differentiation, resulting in expanded skeletal elements and joint fusions. Our results suggest that BMP3 expression in the perichondrium may serve to regulate cartilage cell proliferation by modulating the levels of BMP signaling, thus ensuring proper endochondral ossification.