Stabilization of the tight junction of the intestinal Caco-2 cell monolayer by milk whey proteins.
Stabilization of the tight junction of the intestinal Caco-2 cell monolayer by milk whey proteins.
复制标题
乳清蛋白稳定肠道 Caco-2 细胞单层的紧密连接。
DOI：
10.1271/bbb.59.1951
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Makoto Shimizu
中科院分区：
文献类型：
--
作者：
Kei Hashimoto;Kyoko Takeda;Tsutomu Nakayama;Makoto Shimizu
人肠Caco-2-SF单层的紧密连接（TJ）未稳定形成。通过用β-乳球蛋白（β-Lg）和牛血清白蛋白（BSA）孵育细胞单层来稳定TJ。因此，Caco-2-SF细胞培养系统将为研究TJ稳定因素和研究TJ调节机制提供有用的模型。
The tight junction (TJ) of the human intestinal Caco-2-SF monolayer was shown not to have been stably formed. TJ was stabilized by incubating the cell monolayer with beta-lactoglobulin (beta-Lg) and bovine serum albumin (BSA). Thus, the Caco-2-SF cell-culture system will provide a useful model for studying the factors which stabilize TJ and for investigating the mechanism of TJ regulation.