Toxoplasma gondii: Effects of Artemisia annua L. on susceptibility to infection in experimental models in vitro and in vivo
Toxoplasma gondii: Effects of Artemisia annua L. on susceptibility to infection in experimental models in vitro and in vivo
复制标题
DOI：
10.1016/j.exppara.2009.04.010
复制
发表时间：
2009-07-01
影响因子：
2.1
通讯作者：
Mineo, Jose Roberto
中科院分区：
文献类型：
--
作者：
de Oliveira, Taisa Carrijo;Oliveira Silva, Deise A.;Mineo, Jose Roberto
鉴于弓形虫病的治疗基于药物，但药物副作用大，疗效有限，本研究的目的是评估青蒿对体内和体外刚地弓形虫感染的影响。本研究以干草本植物为原料制备了黄花蒿注射液，并在感染寄生虫的人包皮成纤维细胞（HFF）或小鼠中进行了试验，并与磺胺嘧啶治疗进行了比较。在体外实验中，分别对HFF感染前的寄生虫或之前感染弓形虫的细胞进行处理，并测定每种处理条件下的抑制浓度（IC50）值。在不同浓度的黄花蒿输注和磺胺嘧啶存在下，HFF细胞的存活率都在72%以上，即使两种处理的最高浓度都是如此。此外，在感染HFF细胞之前，用a . annua输注治疗弓形虫速殖子显示出高达75%的抑制量-反应曲线，与用磺胺嘧啶治疗寄生虫的结果相似。体内实验与囊源性弓形虫菌株表明，使用环孢霉输注可以有效地控制感染。总之，我们的研究结果表明，由于其低毒性和直接对寄生虫的抑制作用，青蒿素输注可用于控制弓形虫感染，因此是一种耐受性良好的治疗工具。(C) 2009爱思唯尔公司版权所有。
Considering that the treatment for toxoplasmosis is based on drugs that show limited efficacy due to their substantial side effects, the purpose of the present study was to evaluate the effects of Artemisia annua on in vitro and in vivo Toxoplasma gondii infection. A. annua infusion was prepared from dried herb and tested in human foreskin fibroblasts (HFF) or mice that were infected with the parasite and compared with sulfadiazine treatment. For in vitro experiments, treatment was done on parasite before HFF infection or on cells previously infected with T gondii and the inhibitory concentration (IC50) values for each treatment condition were determined. Viability of HFF cells in the presence of different concentrations of A. annua infusion and sulfadiazine was above 72%, even when the highest concentrations from both treatments were tested. Also, the treatment of T. gondii tachyzoites with A. annua infusion before infection in HFF cells showed a dose-response inhibitory curve that reached up to 75% of inhibition, similarly to the results observed when parasites were treated with sulfadiazine. In vivo experiments with a cystogenic T. gondii strain demonstrated an effective control of infection using A. annua infusion. In conclusion, our results indicate that A. annua infusion is useful to control T gondii infection, due to its low toxicity and its inhibitory action directly against the parasite, resulting in a well tolerated therapeutic tool. (C) 2009 Elsevier Inc. All rights reserved.