Development of a mouse model of arecoline-induced oral mucosal fibrosis
Development of a mouse model of arecoline-induced oral mucosal fibrosis
复制标题
槟榔碱诱导的口腔粘膜纤维化小鼠模型的开发。
DOI：
10.1016/j.apjtm.2017.10.026
复制
发表时间：
2017-12-01
影响因子：
3.1
通讯作者：
Liang, Cui-Wei
中科院分区：
文献类型：
--
作者：
Wen, Qi-Tao;Wang, Tao;Liang, Cui-Wei
目的：为建立槟榔碱诱导的BALB/c小鼠口腔黏膜下纤维性变（oral submucous fibrosis，OSF）模型，观察胶原沉积和血管生成的变化，将BALB/c小鼠随机分为对照组（蒸馏水）和实验组（槟榔碱）。自处理后8周起，每4周处死每组8只小鼠。结果：8周时，口腔黏膜上皮萎缩，固有层内胶原沉积，微血管增生。20周时，舌和腭粘膜结缔组织出现透明变性。结论：高剂量槟榔碱可快速诱导小鼠OSF模型，且随着病程的进展，血管生成和I型胶原的变化有统计学意义（P < 0.05），而III型胶原的变化无统计学意义（P> 0.05）。小鼠OSF血管生成变化主要是减少，胶原蛋白积聚主要是I型胶原蛋白。
Objective: To develop a BALB/c mouse model of oral submucous fibrosis (OSF) induced by arecoline and to exhibit an accumulation of collagen and angiogenesis changes.Methods: BALB/c mice were randomly assigned to either the control (distilled water) or experimental group (arecoline) (n = 40). Eight mice from each group were sacrificed every 4 weeks since 8 weeks post treatment. Changes in histopathologic features, levels of collagen type I and collagen type III, and angiogenesis were measured.Results: In the 8th week, epithelium atrophy, collagen cumulation and micrangium pathologic changes in the lamina propria were observed in the oral mucosa. In the 20th week, hyaline degeneration of the connective tissues was observed on the tongue and palate mucosa. The angiogenesis and collagen type I changed significantly as the diseases advanced (P < 0.05); however, collagen type III was not statistically different.Conclusions: An OSF model involving mice can be rapidly induced by drinking a high-dose of arecoline. OSF angiogenic changes in mice primarily decrease and collagen accumulation is mainly collagen type I.