Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated Arabidopsis hypocotyls
Microtubule bundling plays a role in ethylene-mediated cortical microtubule reorientation in etiolated Arabidopsis hypocotyls
复制标题
微管成束在黄化拟南芥下胚轴乙烯介导的皮质微管重新定向中发挥作用
DOI：
10.1242/jcs.184408
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发表时间：
2016-05-15
影响因子：
4
通讯作者：
Mao, Tonglin
中科院分区：
文献类型：
--
作者：
Ma, Qianqian;Sun, Jingbo;Mao, Tonglin
已知气体激素乙烯在黄化条件下调节植物生长（"三重反应"）。虽然皮层微管的组织是细胞伸长所必需的，但通过激素信号（包括乙烯）调节微管组织的潜在机制尚不明确。在本研究中，我们证明，乙烯信号参与调节皮质微管重定向。特别是，调控微管捆绑是重要的，这一过程中黄化下胚轴。延时分析表明，在各种阵列中形成的微管束结构的选择性稳定与乙烯介导的微管取向有关。捆绑事件和束生长寿命显着增加，在倾斜和纵向阵列，但减少在野生型细胞在响应乙烯的横向阵列。然而，乙烯对微管成束的影响在微管成束蛋白WDL5敲除突变体（wdl5 - 1）中被部分抑制。这项研究表明，调制微管束已形成在某些方向发挥作用，在重新定向微管阵列在乙烯介导的黄化下胚轴细胞伸长。
The gaseous hormone ethylene is known to regulate plant growth under etiolated conditions (the 'triple response'). Although organization of cortical microtubules is essential for cell elongation, the underlying mechanisms that regulate microtubule organization by hormone signaling, including ethylene, are ambiguous. In the present study, we demonstrate that ethylene signaling participates in regulation of cortical microtubule reorientation. In particular, regulation of microtubule bundling is important for this process in etiolated hypocotyls. Time-lapse analysis indicated that selective stabilization of microtubule-bundling structures formed in various arrays is related to ethylene-mediated microtubule orientation. Bundling events and bundle growth lifetimes were significantly increased in oblique and longitudinal arrays, but decreased in transverse arrays in wild-type cells in response to ethylene. However, the effects of ethylene on microtubule bundling were partially suppressed in a microtubule-bundling protein WDL5 knockout mutant (wdl5-1). This study suggests that modulation of microtubule bundles that have formed in certain orientations plays a role in reorienting microtubule arrays in response to ethylene-mediated etiolated hypocotyl cell elongation.