Evidence for distinct vascular and tubular urea transporters in the rat kidney.
Evidence for distinct vascular and tubular urea transporters in the rat kidney.
复制标题
大鼠肾脏中不同血管和管状尿素转运蛋白的证据。
DOI：
--
复制
发表时间：
1996
影响因子：
13.6
通讯作者：
M. Trinh‐Trang‐Tan
中科院分区：
文献类型：
--
作者：
Dominique Promeneur;Germain Rousselet;Lise Bankir;Pascal Bailly;J.;Pierre Ripoche;M. Trinh‐Trang‐Tan
已在几种哺乳动物组织（包括集合管和红细胞）中证实了尿素转运促进。最近已经克隆了两种尿素转运蛋白：UT 2，在兔内髓集合管中表达，和HUT 11，在人红细胞中表达。由于这两种转运蛋白之间的显著同一性（63%），并且由于HUT 11也在人肾脏中表达，因此它们可能代表相同的转运蛋白，但其序列存在种属相关差异。在这里提出的研究中，两个不同的cDNA片段，对应于大鼠的等价物（rUT 2和rUT 11）的两个先前克隆的尿素转运蛋白，通过逆转录-聚合酶链反应分离。这些大鼠探针用于从大鼠组织提取的RNA的北方分析。结果表明，rUT 2和rUT 11是两种不同的尿素转运蛋白：（1）从大鼠体内分离的两个cDNA片段具有不同的序列;（2）rUT 2的mRNA仅在肾脏中发现，有两个转录本。（3.2-和4.4-内切酶（kb）），而rUT 11（只有一个转录本，4.2kb）存在于脑、脾、肾和睾丸中;（3）肾脏外髓内纹表达rUT 11 mRNA和rUT 2短转录本，内髓表达rUT 11和两个rUT 2转录本;（4）在肾小管上皮完全丧失但血管系统完整的积水肾中，rUT 2转录物不再表达，而rUT 11表达增强;（5）实验性慢性尿浓缩活动改变引起rUT 2和rUT 11表达的不同变化。
Facilitated urea transport has been demonstrated in several mammalian tissues, including those of the collecting ducts and red blood cells. Two urea transporters have been recently cloned: UT2, expressed in rabbit inner medullary collecting ducts, and HUT11, expressed in human erythrocytes. Because of significant identity (63%) between these two transporters, and because HUT11 is also expressed in the human kidney, they could represent the same transporter with species-related differences in their-sequences. In the study presented here, two different cDNA fragments, corresponding to the rat equivalents (rUT2 and rUT11) of the two previously cloned urea transporters, were isolated by reverse transcription-polymerase chain reaction. These rat probes were used for Northern analysis of RNA extracted from rat tissues. From the following findings, the results show that rUT2 and rUT11 are two distinct urea transporters: (1) The two cDNA fragments isolated in the rat exhibit different sequences; (2) The mRNA for rUT2 is found exclusively in the kidney, with two transcripts (3.2- and 4.4-kilobase (kb)), whereas rUT11 (only one transcript, 4.2 kb) is present in the brain, spleen, kidney, and testis; (3) in the kidney, the inner stripe of the outer medulla expresses rUT11 mRNA and the short transcript of rUT2, whereas the inner medulla expresses rUT11 and the two rUT2 transcripts; (4) In hydronephrotic kidneys that have completely lost their tubular epithelium but have intact vasculature, rUT2 transcripts are no longer expressed, whereas expression of rUT11 is intensified; (5) Experimental chronic alterations in urine concentrating activity induced different changes in the expression of rUT2 and rUT11.
登录
查看更多内容
DOI：
--
发表时间：
1992-06
期刊：
Journal of the American Society of Nephrology : JASN
影响因子：
--
作者：
J. Sands;J. Gargus;O. Fröhlich;R. Gunn;J. Kokko
通讯作者：
J. Sands;J. Gargus;O. Fröhlich;R. Gunn;J. Kokko
影响因子：
--
作者：
PALLONE, TL
通讯作者：
PALLONE, TL
非洲爪蟾卵母细胞中 cAMP 依赖性和非依赖性尿素转运蛋白的功能表达。
DOI：
10.1152/ajpcell.1993.265.2.c514
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Hasegawa,H;Verkman,AS
通讯作者：
Verkman,AS
影响因子：
15.9
作者：
SMITH, CP;LEE, WS;HEDIGER, MA
通讯作者：
HEDIGER, MA