Differences in RNA patterns of influenza A viruses
Differences in RNA patterns of influenza A viruses
复制标题
甲型流感病毒RNA模式的差异
作者：
P. Palese;J. Schulman
通过聚丙烯酰胺尿素平板凝胶电泳分析甲型流感病毒的节段RNA，提供了一种比以前更清晰地分辨不同节段的数量和迁移率的方法。使用该系统，流感A/WSN（HON 1）病毒的RNA基因组可以被分离成7至9个单独的条带，这取决于病毒是在高或低多重感染后获得的，流感A/PR/8（HON 1）病毒的基因组可以被解析成8个条带，其中6个条带与WSN病毒的可比RNA条带不同地迁移。由流感A/PR/8（HON 1）和A/England/42/72（H8 n2）病毒产生的RNA模式的比较也揭示了不同条带的迁移速度的主要差异，并且对源自这两种毒株的HON 2重组病毒的RNA的RNA的分析允许鉴定源自一个特定亲本的RNA片段。通过这些技术的扩展，有可能确定每种病毒蛋白质的RNA片段编码，并分析重组株，以确定哪些基因来源于其亲本。
Analysis of the segmented RNAs of influenza A viruses by electrophoresis on polyacrylamide urea slab gels has provided a method for sharper resolution of the number and migration rates of different segments than previously has been possible. Using this system, the RNA genome of influenza A/WSN (HON1) virus can be separated into seven to nine separate bands, depending on whether virus is obtained after high or low multiplicity of infection, and the genome of influenza A/PR/8 (HON1) virus can be resolved into eight bands, six of which migrate differently from comparable RNA bands of WSN virus. Comparision of the RNA patterns produced by influenza A/PR/8 (HON1) and A/England/42/72 (H8n2) virus also reveals major differences in migration speeds of different bands, and analysis of the RNAs of the RNAs of an HON2 recombinant virus derived from these two strains permits the identification of RNA segments which have been derived from one particular parent. By extension of these techniques, it may be possible to define which RNA segment codes for each viral protein and to analyze recombinant strains to identify which genes have been derived from each of its parents.