Strain-Dependent Norovirus Bioaccumulation in Oysters
Strain-Dependent Norovirus Bioaccumulation in Oysters
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DOI：
10.1128/aem.03010-10
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发表时间：
2011-05-01
影响因子：
4.4
通讯作者：
Le Guyader, Francoise S.
中科院分区：
文献类型：
--
作者：
Maalouf, Haifa;Schaeffer, Julien;Le Guyader, Francoise S.
诺如病毒是人类胃肠炎的主要病原体，也是贝类疾病暴发的主要病原体。一些NoV菌株通过使用与其人类配体类似的碳水化合物结构与贝类组织结合，导致假设这些配体可能影响生物累积。本研究比较了来自两个主要人类诺如病毒基因组的三株病毒在牡蛎（Crassostreagigas）中的生物富集效率和组织分布。观察到菌株之间存在明显差异。GI. 1菌株是最有效浓缩的菌株。生物积累具体发生在消化组织中的剂量依赖性的方式，其效率的配体表达，这是最高的在寒冷的月份。相比之下，GII.4菌株的生物累积性非常差，几乎在所有组织中均可回收，无季节性影响。GII.3菌株表现出一种中间行为，没有季节性影响，在寒冷的月份，生物累积效率低于GI.1菌株。此外，GII.3菌株在消化组织中几乎特异性积累之前在鳃和外套膜中短暂富集。菌株的碳水化合物配体特异性至少部分解释了菌株依赖性生物累积特性。特别是，结合消化管特异性配体应有助于生物积累，而我们假设，结合唾液酸的配体存在于所有组织将有助于保留病毒颗粒在鳃或外套膜，并导致快速破坏。
Noroviruses (NoVs) are the main agents of gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Some NoV strains bind to shellfish tissues by using carbohydrate structures similar to their human ligands, leading to the hypothesis that such ligands may influence bioaccumulation. This study compares the bioaccumulation efficiencies and tissue distributions in oysters (Crassostrea gigas) of three strains from the two principal human norovirus genogroups. Clear differences between strains were observed. The GI.1 strain was the most efficiently concentrated strain. Bioaccumulation specifically occurred in digestive tissues in a dose-dependent manner, and its efficiency paralleled ligand expression, which was highest during the cold months. In comparison, the GII.4 strain was very poorly bioaccumulated and was recovered in almost all tissues without seasonal influence. The GII.3 strain presented an intermediate behavior, without seasonal effect and with less bioaccumulation efficiency than that of the GI.1 strain during the cold months. In addition, the GII.3 strain was transiently concentrated in gills and mantle before being almost specifically accumulated in digestive tissues. Carbohydrate ligand specificities of the strains at least partly explain the strain-dependent bioaccumulation characteristics. In particular, binding to the digestive-tube-specific ligand should contribute to bioaccumulation, whereas we hypothesize that binding to the sialic acid-containing ligand present in all tissues would contribute to retain virus particles in the gills or mantle and lead to rapid destruction.