Characteristics and Growth Patterns of Human Peritoneal Mesothelial Cells: Comparison Between Advanced Epithelial Ovarian Cancer and Non-Ovarian Cancer Sources
Characteristics and Growth Patterns of Human Peritoneal Mesothelial Cells: Comparison Between Advanced Epithelial Ovarian Cancer and Non-Ovarian Cancer Sources
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DOI：
10.1177/107155769900600610
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发表时间：
1999-11
期刊：
影响因子：
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通讯作者：
Xiangyun Zhang;R. Pettengell;N. Nasiri;V. Kalia;A. Dalgleish;D. Barton
中科院分区：
文献类型：
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作者：
Xiangyun Zhang;R. Pettengell;N. Nasiri;V. Kalia;A. Dalgleish;D. Barton
目的：比较晚期上皮性卵巢癌（EOC）患者与非 EOC 患者腹膜间皮细胞（HPMC）的特征和生长模式。方法：从初治患者身上获取腹膜和大网膜活检组织。对福尔马林固定活检和细胞学接触制剂进行了免疫化学研究。 HPMCs 从组织活检或恶性腹水中分离出来，并在有或没有生长因子的情况下培养。通过MTT测定测定细胞生长。通过流式细胞术分析 (FACS) 进一步表征培养的 HPMC。结果：腹膜活检显示非 EOC 患者具有连续的扁平间皮细胞层，而晚期 EOC 患者的间皮是不连续的圆形细胞层。在所有腹膜活检中，间皮表达细胞角蛋白 8/18、波形蛋白和间皮瘤细胞抗原，但不表达 E-钙粘蛋白。在接触制剂中，推定的成纤维细胞抗原的表达范围从阴性到弱阳性。非 EOC 病例的 HPMC 在体外生长缓慢，除非在培养的最初 24 小时内暴露于 L-半胱氨酸 (L-cys 30 μg/mL)。相反，来自 EOC 来源的细胞生长得更快，特别是当同时暴露于表皮生长因子 (EGF 10 ng/mL) 和氢化可的松 (HC 400 ng/mL) 时。 HPMC在体外共表达细胞角蛋白8/18和波形蛋白，但假定的成纤维细胞抗原的表达在原代培养期间增加，而间皮瘤细胞抗原的表达在连续传代中减少。此外，在 FACS 中，培养的 HPMC 不表达 CD14、CD16 或 CD34。结论：在非EOC和EOC患者的腹膜活检中，HPMC表现出不同的形态，但免疫染色特征相似，而不同来源的培养细胞在形态和表型上相似。 L-半胱氨酸促进非 EOC 的生长，但不促进 EOC 衍生的 HPMC 的生长，后者对 EGF 和 HC 的反应最大。来自 EOC 的 HPMC 的体外生长优势表明这些细胞处于启动或激活状态。
Objective: To compare the characteristics and growth patterns of human peritoneal mesothelial cells (HPMCs) from advanced epithelial ovarian cancer (EOC) patients with those from non-EOC patients. Methods: Peritoneal and omental biopsies were obtained from treatment-naïve patients. Formalin-fixed biopsies and cytologic touch preparations were studied immunochemically. HPMCs were isolated from tissue biopsies or malignant ascites and cultured with or without growth factors. Cell growth was determined by the MTT assay. Cultured HPMCs were further characterised by flow cytometry analysis (FACS). Results: Peritoneal biopsies showed a continuous flat mesothelial cell layer in non-EOC patients, whereas in advanced EOC, the mesothelium was a discontinuous layer of rounded cells. In all peritoneal biopsies, the mesothelium expressed cytokeratin 8/18, vimentin, and the mesothelioma cell antigen but not E-cadherin. In touch preparations, expression of the putative fibroblast antigen ranged from negative to weakly positive. HPMC from non-EOC cases grew slowly in vitro except when exposed to L-cysteine (L-cys 30 μg/mL) during the initial 24 hours of culture. Conversely, cells from EOC sources grew more rapidly, especially when exposed to both epidermal growth factor (EGF 10 ng/mL) and hydrocortisone (HC 400 ng/mL). HPMC coexpressed cytokeratin 8/18 and vimentin in vitro, but the expression of the putative fibroblast antigen increased during primary culture, whereas that of the mesothelioma cell antigen decreased in successive passages. Furthermore, in FACS, cultured HPMC did not express CD14, CD16, or CD34. Conclusion: In peritoneal biopsies from non-EOC and EOC patients, HPMCs showed different morphology but similar immunostaining characteristics, whereas cultured cells from different sources were similar in both morphology and phenotype. L-cysteine enhanced the growth of non-EOC but not of EOC-derived HPMCs, which had a maximal response to EGF and HC. The growth advantage of HPMCs from EOC in vitro suggests these cells are in a primed or activated state.