Insights into ecological role of a new deltaproteobacterial order Candidatus Acidulodesulfobacterales by metagenomics and metatranscriptomics
Insights into ecological role of a new deltaproteobacterial order Candidatus Acidulodesulfobacterales by metagenomics and metatranscriptomics
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通过宏基因组学和宏转录组学深入了解新的δ变形菌目 Candidatus Acidulodesulfobacterales 的生态作用
DOI：
10.1038/s41396-019-0415-y
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Shu Wen Sheng
中科院分区：
文献类型：
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作者：
Tan Sha;Liu Jun;Fang Yun;Hedlund Brian P;Lian Zheng Han;Huang Li Ying;Li Jin Tian;Huang Li Nan;Li Wen Jun;Jiang Hong Chen;Dong Hai Liang;Shu Wen Sheng
一些丰富但尚未培养的细菌群存在于极端的铁和硫丰富的环境中，这些细菌的生理学，生物多样性和生态作用仍然是一个谜。在这里，我们检索了四个宏基因组组装的基因组（MAG）从人工酸性矿山排水（AMD）系统，并提出他们属于一个新的deltaproteobacterial命令，Aciduloacidifobacterales。Ca.中国东南部AMD中的嗜酸杆菌与亚铁有很强的相关性。重建的代谢途径和基因表达谱表明，他们可能是兼性厌氧自养固氮能力。除了由dsrAB、dsrD、dsrL和dsrEFH基因编码的异化硫酸盐还原外，这些微生物还可能氧化硫化物，这取决于氧浓度和/或氧化还原电位。还鉴定了与参与铁代谢的基因具有同源性的几个基因，表明它们在铁循环中的潜在作用。此外，丰富的抗性基因的表达揭示了这些生物在AMD环境中对极端环境胁迫的适应和响应机制。这些发现揭示了分布，多样性和潜在的生态作用的新秩序Ca。自然界中的嗜酸杆菌。
Several abundant but yet uncultivated bacterial groups exist in extreme iron- and sulfur-rich environments, and the physiology, biodiversity, and ecological roles of these bacteria remain a mystery. Here we retrieved four metagenome-assembled genomes (MAGs) from an artificial acid mine drainage (AMD) system, and propose they belong to a new deltaproteobacterial order, Candidatus Acidulodesulfobacterales. The distribution pattern of Ca. Acidulodesulfobacterales in AMDs across Southeast China correlated strongly with ferrous iron. Reconstructed metabolic pathways and gene expression profiles showed that they were likely facultatively anaerobic autotrophs capable of nitrogen fixation. In addition to dissimilatory sulfate reduction, encoded by dsrAB, dsrD, dsrL, and dsrEFH genes, these microorganisms might also oxidize sulfide, depending on oxygen concentration and/or oxidation reduction potential. Several genes with homology to those involved in iron metabolism were also identified, suggesting their potential role in iron cycling. In addition, the expression of abundant resistance genes revealed the mechanisms of adaptation and response to the extreme environmental stresses endured by these organisms in the AMD environment. These findings shed light on the distribution, diversity, and potential ecological role of the new order Ca. Acidulodesulfobacterales in nature.