The effect of shock pressures on the inactivation of a marine Vibrio sp.
The effect of shock pressures on the inactivation of a marine Vibrio sp.
复制标题
DOI：
10.1007/s00193-007-0089-7
复制
发表时间：
2007-06
期刊：
影响因子：
2.2
通讯作者：
A. Abe;H. Mimura;H. Ishida;Kazutoshi Yoshida
中科院分区：
文献类型：
--
作者：
A. Abe;H. Mimura;H. Ishida;Kazutoshi Yoshida
冲击压力对海洋弧菌失活的影响。进行了实验和数值研究。在实验中，采用气枪加速的铝冲击板，在密闭的铝容器内诱发细胞悬浮液的冲击波。用压膜计测量容器内的冲击压力。在激波前测量了几个10 - 100mpa的压力。采用固体铝的Johnson-Cook模型和悬浮液的Tait方程，对铝容器内冲击压力的产生机理进行了有限元模拟。通过数值模拟，合理地预测了激波在铝容器内固液边界处的反射、衍射和相互作用。计算模拟得到的冲击压力变化与实验结果吻合较好。随着激波峰值压力的增大，活细胞数量逐渐减少。峰值压力高于200mpa时，细胞完全失活。在这种压力下，细胞结构会像红细胞一样变形，一些蛋白质会从细胞中泄漏出来。这些结果表明，冲击波产生的正负压波动有助于海洋弧菌的失活。
The effect of shock pressures on the inactivation of a marineVibriosp. was studied experimentally and numerically. In the experiment, an aluminum impactor plate accelerated by a gas gun was used to induce shock waves in a sealed aluminum container with cell suspension liquid inside. The shock pressures in the container were measured by a piezofilm gauge. Several 10–100 MPa of pressure were measured at the shock wave front. An FEM simulation, using the Johnson–Cook model for solid aluminum and the Tait equation for the suspension liquid, was carried out in order to know the generation mechanism of shock pressures in the aluminum container. The reflection, diffraction and interaction of shock waves at the solid–liquid boundaries in the aluminum container were reasonably predicted by the numerical simulation. The changes in shock pressures obtained from the computational simulation were in good agreement with those from the experiment. The number of viable cells decreased with the increase of peak pressures of the shock waves. Peak pressures higher than 200 MPa completely inactivated the cells. At this pressure, the cell structures were deformed like the shape of red blood cells, and some proteins leaked from the cells. These results indicate that the positive and negative pressure fluctuations generated by shock waves contribute to the inactivation of the marineVibriosp.