Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome.
Physical and genetic characterization of the glnA--glnG region of the Escherichia coli chromosome.
复制标题
大肠杆菌染色体 glnA--glnG 区域的物理和遗传特征。
DOI：
10.1073/pnas.78.6.3743
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发表时间：
1981
影响因子：
11.1
通讯作者：
Magasanik,B
中科院分区：
文献类型：
--
作者：
Backman,K;Chen,YM;Magasanik,B
我们已经克隆并表征了大肠杆菌染色体的跨越glnA的片段，glnA是谷氨酰胺合成酶（L-谷氨酸：氨连接酶（ADP-形成），EC 6.3.1.2）的结构基因。该片段还携带glnG，其产物是调节glnA表达所必需的，以及一个先前未鉴定的基因，其功能我们尚未发现。glnA和新鉴定的基因的转录从两个基因之间的区域发散地发生。glnA的转录朝向glnG进行，glnG以相同的方向转录。glnA和glnG之间的DNA区域包含遗传信息，其丢失可能导致不能响应氨而降低glnA和其他操纵子的表达（GlnC表型）。
We have cloned and characterized a fragment of the Escherichia coli chromosome spanning glnA, the structural gene for glutamine synthetase (L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2]. The fragment also carriers glnG, whose product is necessary for regulation of glnA expression, and a previously unidentified gene whose function we have not discovered. Transcription of glnA and the newly identified gene occurs divergently from a region between the two genes. Transcription of glnA proceeds toward glnG, which is transcribed in the same direction. A region of DNA between glnA and glnG contains genetic information whose loss may result in the inability to reduce expression of glnA and other operons in response to ammonia (the GlnC phenotype).