Increased expression of genes that control ionic homeostasis, second messenger signaling and metabolism in the carotid plaques from patients with symptomatic stroke
Increased expression of genes that control ionic homeostasis, second messenger signaling and metabolism in the carotid plaques from patients with symptomatic stroke
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DOI：
10.1111/j.1471-4159.2005.03516.x
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发表时间：
2006-04-01
影响因子：
4.7
通讯作者：
Dempsey, RJ
中科院分区：
文献类型：
--
作者：
Vemuganti, R;Dempsey, RJ
导致颈动脉粥样硬化斑块出现症状的分子机制尚未确定。使用 Affymetrix 人类基因芯片组，我们分析了 6 名有症状中风患者和 4 名无症状患者手术切除的颈动脉斑块中 44 862 个 mRNA 转录物的基因表达模式。两组的年龄、体重指数和狭窄程度相似。大约 236 个转录本（相当于分析的总转录本的 0.5%）在有症状组中的表达量比无症状组更丰富。其中，61 个转录本参与离子稳态、信号转导和代谢。在症状斑块中上调的其他转录本包括癌基因、生长因子、肿瘤标志物、血管生成促进剂、转录因子以及RNA剪接和加工因子。这项研究表明，一些动脉粥样硬化斑块中较高的代谢活性导致其更快的生长和中风症状的沉淀。这些发现的意义在于，中风症状的诊断和预防可能在基因水平上成为可能。
The molecular mechanisms that render a carotid atherosclerotic plaque symptomatic have not yet been identified. Using an Affymetrix Human GeneChip set, we analyzed the gene expression patterns of 44 862 mRNA transcripts in surgically removed carotid artery plaques from six patients with symptomatic stroke and four non-symptomatic patients. The age, body mass index and the degree of stenosis were similar in the two groups. Some 236 transcripts (similar to 0.5% of the total transcripts analyzed) were expressed more abundantly in the symptomatic than the asymptomatic group. Of these, 61 transcripts are those that participate in ionic homeostasis, signal transduction and metabolism. The other groups of transcripts up-regulated in the symptomatic plaques include oncogenes, growth factors, tumor markers, angiogenesis promoters, transcription factors, and RNA splicing and processing factors. This study indicates that the higher metabolic activity in some atherosclerotic plaques leads to their faster growth and precipitation of stroke symptoms. The implications of these findings are that both diagnosis and prevention of stroke symptoms may become possible at the genetic level.