Evaluation of cartilage degradation in arthritis using T1ρ magnetic resonance imaging mapping
Evaluation of cartilage degradation in arthritis using T1ρ magnetic resonance imaging mapping
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DOI：
10.1007/s00296-011-2140-3
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发表时间：
2012-09-01
影响因子：
4
通讯作者：
Iwamoto, Yukihide
中科院分区：
文献类型：
--
作者：
Tsushima, Hidetoshi;Okazaki, Ken;Iwamoto, Yukihide
T1 rho磁共振成像（MRI）可用于绘制软骨中蛋白多糖（PG）的损失。在这里，我们使用T1 rho MRI来绘制骨关节炎（OA）和类风湿性关节炎（RA）的软骨退化图。从5例RA患者和14例OA患者全膝关节置换术（TKA）后获得组织样本。测量三个参数：首先，对软骨样本组织进行宏观分级，分为5个等级（G0：正常，G1：肿胀，G2：浅表纤颤，G3：深纤颤，G4：软骨下骨暴露）。其次，通过测量数码拍摄的safranin - o染色石蜡切片的光密度来评估PG的半定量值。第三，将软骨分为浅层和深层，量化T1 rho值。各组间浅层OA、RA T1 rho值差异有统计学意义（OA组为G0/1、G0/2； RA组为G0/2、G1/2）。在深层，OA和RA的T1 rho值在组间也有显著差异。在浅层和深层，平均T1 rho值与宏观分级之间存在显著相关性（OA P < 0.01, RA P < 0.001）。我们发现Safranin-O染色评分与T1 rho值呈负相关（OA r = -0.61, RA r = -0.79）。此外，RA受试者的T1 rho值明显高于形态等级相似的OA受试者。总之，T1 rho MRI能够检测和绘制OA和RA软骨退化的早期阶段。该方法可靠，可用于评估关节炎软骨的大分子变化。
T1 rho magnetic resonance imaging (MRI) can be used to map proteoglycan (PG) loss in cartilage. Here, we used T1 rho MRI to map cartilage degradation in osteoarthritis (OA) and rheumatoid arthritis (RA). Tissue samples were obtained from five RA patients and 14 OA patients following total knee arthroplasty (TKA). Three parameters were measured: First, macroscopic grading of cartilage sample tissues was performed on a 5-grade scale (G0: normal, G1: swelling, G2: superficial fibrillation, G3: deep fibrillation, G4: subchondral bone exposure). Second, semi-quantitative values of PG were assessed by measuring the optical density of Safranin-O-stained paraffin sections that had been digitally photographed. Third, cartilage was divided into superficial and deep layers and the T1 rho values were quantified. T1 rho values of OA and RA in the superficial layers showed significant differences between groups (G0/1 and G0/2 for OA; G0/2 and G1/2 for RA). In the deep layers, T1 rho values of OA and RA also differed significantly between groups. In both the superficial and deep layers, there was a significant correlation between the mean T1 rho values and macroscopic grading (P < 0.01 for OA, P < 0.001 for RA). We found a negative correlation between the score of Safranin-O staining and T1 rho values (r = -0.61 for OA, r = -0.79 for RA). In addition, RA subjects had significantly higher T1 rho values than OA subjects of similar morphologic grade. In conclusion, T1 rho MRI is able to detect and map the early stages of cartilage degradation in OA and RA. This method is reliable and useful for the evaluation of macromolecular changes in arthritic cartilage.