Real-time hexose monophosphate shunt activity in light- and dark-adapted rabbit retinas.
Real-time hexose monophosphate shunt activity in light- and dark-adapted rabbit retinas.
复制标题
光适应和暗适应兔视网膜中的实时单磷酸己糖分流活性。
DOI：
10.1159/000265511
复制
发表时间：
1987
影响因子：
2.1
通讯作者：
Lam,DM
中科院分区：
文献类型：
--
作者：
Aguiar,E;Cheng,HM;Lam,DM
相关质子核磁共振波谱在明适应和暗适应的兔视网膜上进行，以阐明实时己糖一磷酸分流(HMPS)的活性。光最初显著刺激视网膜HMPS，其消耗的葡萄糖比暗适应的视网膜多17%。在光照60分钟后，葡萄糖消耗量最终下降到总消耗量的16%，即基线水平。相比之下，暗适应视网膜的HMPS活性最初占总葡萄糖消耗量的29%，40分钟后降至基础水平。在这两组视网膜中，乳酸的产生似乎都很稳定。叔丁基氢过氧化氢(1 MM)也能刺激分流；然而，光适应和叔丁基氢过氧化氢的结合不能额外地刺激分流。这些数据表明，视网膜的HMPS容量有限。
Correlation proton nuclear magnetic resonance spectroscopy was performed on light- and dark-adapted rabbit retinas to elucidate real-time hexose monophosphate shunt (HMPS) activity. Light significantly stimulated retinal HMPS initially, which consumed 17% more glucose than that in dark-adapted retinas. The glucose consumption eventually declined to 16% of total, the baseline level, after 60 min of light exposure. In contrast, dark-adapted retinas showed an initial HMPS activity of 29% total glucose consumption, which declined to the basal level after 40 min. Lactate production appeared stable in both sets of retinas. Tert-butyl hydroperoxide (1 mM) also stimulated the shunt; however, a combination of light-adaptation and tert-butyl hydroperoxide did not stimulate the shunt additively. These data indicate that the retina has limited HMPS capacity.