A new class of retroviral and satellite encoded small RNAs emanates from mammalian centromeres
A new class of retroviral and satellite encoded small RNAs emanates from mammalian centromeres
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DOI：
10.1007/s00412-008-0181-5
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发表时间：
2009-02-01
期刊：
影响因子：
1.6
通讯作者：
O'Neill, Rachel J.
中科院分区：
文献类型：
--
作者：
Carone, Dawn M.;Longo, Mark S.;O'Neill, Rachel J.
真核生物着丝粒核心的转录框架已经在芽殖酵母和水稻中描述，但对于大多数真核生物和所有脊椎动物，它仍然是未知的。在tammar小袋鼠缺乏大的着丝粒间重复序列，使得首次在哺乳动物物种中定位和鉴定着丝粒处的转录单位成为可能。我们表明，这些转录单位，卫星和逆转录病毒组成，结合着丝粒蛋白，它们是一类新的小RNA的来源。现在已知这些小RNA来源的内源性逆转录病毒位于几种脊椎动物的着丝粒结构域中。这种新的RNA形式的发现汇集了几个独立的证据，指向一个保守的逆转录病毒编码的真核着丝粒内的加工RNA实体。
The transcriptional framework of the eukaryotic centromere core has been described in budding yeast and rice, but for most eukaryotes and all vertebrates it remains largely unknown. The lack of large pericentric repeats in the tammar wallaby has made it possible to map and identify the transcriptional units at the centromere in a mammalian species for the first time. We show that these transcriptional units, comprised of satellites and a retrovirus, are bound by centromere proteins and that they are the source of a novel class of small RNA. The endogenous retrovirus from which these small RNAs are derived is now known to be in the centromere domain of several vertebrate classes. The discovery of this new RNA form brings together several independent lines of evidence that point to a conserved retroviral-encoded processed RNA entity within eukaryotic centromeres.