Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model.
Application of optically-induced-dielectrophoresis in microfluidic system for purification of circulating tumour cells for gene expression analysis- Cancer cell line model.
复制标题
在微流体系统中使用光学诱导的二卫生到循环肿瘤细胞中用于基因表达分析 - 癌细胞系模型。
DOI：
10.1038/srep32851
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发表时间：
2016-09-09
影响因子：
4.6
通讯作者：
Wu MH
中科院分区：
文献类型：
--
作者：
Chiu TK;Chou WP;Huang SB;Wang HM;Lin YC;Hsieh CH;Wu MH
血液循环系统中的循环肿瘤细胞（CTCs）与癌症转移有关。对癌症患者CTCs耐药基因表达的分析有望为个体患者选择更有效的治疗方案。然而，目前的CTC分离方案可能无法获得足够高纯度的CTC用于此类应用。针对这一问题，本研究提出将光学诱导介电泳（ODEP）力控细胞操作技术和荧光显微成像技术整合到微流控系统中，在常规CTC分离方法之后进一步纯化CTC。本研究开发了微流控系统，并对其分离CTC的最佳操作条件和性能进行了评价。结果表明，该系统能够以高达100%的细胞纯度分离ctc，超出了使用先前现有技术的可能性。因此，在分析CTC基因表达时，该方法可以排除细胞样本中白细胞的干扰，从而具有更高的分析灵敏度，如本研究所示。总的来说，本研究提出了一种基于odep的微流体系统，能够简单有效地从细胞混合物中分离出特定的细胞种类。
Circulating tumour cells (CTCs) in a blood circulation system are associated with cancer metastasis. The analysis of the drug-resistance gene expression of cancer patients’ CTCs holds promise for selecting a more effective therapeutic regimen for an individual patient. However, the current CTC isolation schemes might not be able to harvest CTCs with sufficiently high purity for such applications. To address this issue, this study proposed to integrate the techniques of optically induced dielectrophoretic (ODEP) force-based cell manipulation and fluorescent microscopic imaging in a microfluidic system to further purify CTCs after the conventional CTC isolation methods. In this study, the microfluidic system was developed, and its optimal operating conditions and performance for CTC isolation were evaluated. The results revealed that the presented system was able to isolate CTCs with cell purity as high as 100%, beyond what is possible using the previously existing techniques. In the analysis of CTC gene expression, therefore, this method could exclude the interference of leukocytes in a cell sample and accordingly contribute to higher analytical sensitivity, as demonstrated in this study. Overall, this study has presented an ODEP-based microfluidic system capable of simply and effectively isolating a specific cell species from a cell mixture.
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影响因子：
9.3
作者：
Kuhlmann, Jan Dominik;Wimberger, Pauline;Kasimir-Bauer, Sabine
通讯作者：
Kasimir-Bauer, Sabine
影响因子：
6.1
作者：
Huang, Song-Bin;Wu, Min-Hsien;Lee, Gwo-Bin
通讯作者：
Lee, Gwo-Bin
DOI：
10.1186/bcr2131
发表时间：
2008
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Deng G;Herrler M;Burgess D;Manna E;Krag D;Burke JF
通讯作者：
Burke JF
影响因子：
6.4
作者：
Nadal, Rosa;Gabriel Ortega, F.;Jose Serrano, M.
通讯作者：
Jose Serrano, M.
影响因子：
11.5
作者：
Danila, Daniel C.;Heller, Glenn;Scher, Howard I.
通讯作者：
Scher, Howard I.