Artificial chromosome preparation in arabidopsis.
Artificial chromosome preparation in arabidopsis.
复制标题
拟南芥人工染色体制备。
DOI：
10.1002/cppb.20010
复制
发表时间：
2016
影响因子：
--
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Murata;M.
在拟南芥中，已经进行了各种尝试来创造人造染色体，作为细胞学和遗传学分析的新工具。然而，直到最近，大多数努力都没有成功。大多数真核细胞的染色体是线性的，因此拟南芥的人工染色体也被设计成线性的，并在两端携带端粒结构。相反，Cre/LoxP系统与Ac/DS转座子系统相结合，在发现环状微染色体相对稳定并可遗传给下一代Ina的基础上，成功地创造了环形人工染色体。塔里安娜。由于已经产生了1-6Mb的环状微染色体∼，本文描述了诱导大的染色体重排导致环状染色体形成的方案。2016John Wiley&Sons，Inc.
InArabidopsis thaliana, various attempts have been made to create artificial chromosomes as a new tool for cytological and genetic analyses. However, most of the efforts have been unsuccessful until recently. Most eukaryotic chromosomes are linear, and therefore the Arabidopsis artificial chromosomes have also been designed to be linear and to carry the telomere structure at both ends. In contrast, circular artificial chromosomes were successfully created by the Cre/LoxPsystem combined with Ac/Ds transposon system, on the basis of the discovery that ring minichromosomes are relatively stable and transmissible to the next generations inA. thaliana. Because ring minichromosomes ∼1 to 6 Mb in size have been generated, in this article, the protocol for inducing large chromosomal rearrangements resulting in ring chromosome formation is described. © 2016 by John Wiley & Sons, Inc.