Antigenic relatedness between Burkholderia pseudomallei and Burkholderia mallei
Antigenic relatedness between Burkholderia pseudomallei and Burkholderia mallei
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DOI：
10.1111/j.1348-0421.2002.tb02679.x
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发表时间：
2002-01-01
影响因子：
2.6
通讯作者：
Sirisinha, S
中科院分区：
文献类型：
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作者：
Anuntagool, N;Sirisinha, S
类鼻疽伯克霍尔德菌和鼻疽伯克霍尔德菌分别是不同疾病的病原体，即类鼻疽病和鼻疽。根据DNA-DNA同源性、16 S rRNA碱基序列和表型特征，这两个物种非常密切相关。在使用多克隆抗血清的基础上，B.类鼻疽和B.还发现鼻疽在抗原性上彼此密切相关。我们以前曾报道过抗B的单克隆抗体（MAbs）的产生。类鼻疽抗原;一组对所有B表面上存在的200-kDa胞外多糖具有特异性。另一种对存在于B的95%以上的脂多糖（LPS）结构具有特异性。检测了假鼻疽。在本研究中，我们发现，单克隆抗体抗200 kDa抗原的B。假鼻疽与也存在于某些B中的组分发生交叉反应。鼻疽分离株（3/6），但免疫印迹反应在200-kDa位置以下。另一方面，六个B。鼻疽分离株与B特异性的MA B反应。类鼻疽LPS。有趣的是，观察到只有3个外泌多糖阳性B。鼻疽菌分离株与抗B的商业MA B反应。鼻疽LPS。所提供的数据表明，B。鼻疽可以基于它们与不同MAb的反应性在抗原上分为两种类型，即，外泌多糖的存在或不存在以及脂多糖的类型。来自这两种密切相关的生物体的LPS的异质性最可能与其O-多糖侧链的差异有关。
Burkholderia pseudomallei and Burkholderia mallei are causative agents of distinct diseases, namely, melioidosis and glanders, respectively. The two species are very closely related, based on DNA-DNA homology, base sequence of the 16S rRNA, and phenotypic characteristics. Based on the use of polyclonal antisera, B. pseudomallei and B. mallei are also found to be antigenically closely related to one another. We previously reported the production of monoclonal antibodies (MAbs) against B. pseudomallei antigens; one group was specific for the 200-kDa exopolysaccharide present on the surface of all B. pseudomallei isolates, and the other was specific for the lipopolysaccharide (LPS) structure present on more than 95% of the B. pseudomallei tested. In the present study, we showed that the MAbs against 200-kDa antigen of B. pseudomallei cross-reacted with a component present also in some B. mallei isolates (3/6), but the positive immunoblot reaction was noted below the 200-kDa position. On the other hand, none of the six B. mallei isolates reacted with the MAb specific for B. pseudomallei LPS. It was of interest to observe that only the 3 exopolysaccharide-positive B. mallei isolates reacted with a commercial MAb against B. mallei LPS. The data presented suggest that B. mallei can be classified antigenically into two types based on their reactivities with different MAbs, i.e., the presence or absence of exopolysaccharide and the types of lipopolysaccharide. The heterogeneity of the LPS from these two closely related organisms is most likely related to the differences in its O-polysaccharide side chain.