Expression pattern and activity of six glutelin gene promoters in transgenic rice.
Expression pattern and activity of six glutelin gene promoters in transgenic rice.
复制标题
转基因水稻中六个麸质蛋白基因启动子的表达模式和活性。
DOI：
10.1093/jxb/ern110
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发表时间：
2008
影响因子：
6.9
通讯作者：
Song, Yan Ru
中科院分区：
文献类型：
--
作者：
Qu, Le Qing;Xing, Yan Ping;Liu, Wen Xian;Xu, Xiu Ping;Song, Yan Ru
缺乏强大的胚乳特异性表达启动子来驱动重组蛋白基因在谷物胚乳中的表达，这是获得所需表达水平和表达模式的主要限制。采用PCR方法从水稻（Oryza sativa L.）基因组DNA中分离出6个种子贮藏谷蛋白基因启动子（GluA-1、GluA-2、GluA-3、GluB-3、GluB-5和GluC）。以β-葡萄糖醛酸酶（GUS）为报告基因，研究了它们在水稻稳定转基因植株中的时空表达模式和表达潜力。所有启动子在胚乳内均表现出预期的空间表达。GluA-1、GluA-2和GluA-3启动子主要在胚乳外侧（外周区）调控GUS的表达。GluB-5和GluC启动子引导GUS在整个胚乳中的表达，后者在整个胚乳中几乎均匀表达，这与其他水稻谷蛋白基因启动子的特点不同。GluB-3启动子仅在糊粉层和亚糊粉层中指导GUS的表达。在种子成熟过程中对启动子活性的检测表明，GluC启动子比其他启动子具有更高的活性。这些启动子是在单子叶胚乳中实现多目的基因表达的理想候选子，但避免了基于启动子同源性的基因沉默。GluC启动子不含胚乳特异性决定基序GCN4、AACA和prolamin-box，这表明在决定胚乳特异性方面存在额外的调控机制。
The shortage of strong endosperm-specific expression promoters for driving the expression of recombinant protein genes in cereal endosperm is a major limitation in obtaining the required level and pattern of expression. Six promoters of seed storage glutelin genes (GluA-1, GluA-2, GluA-3, GluB-3, GluB-5, and GluC) were isolated from rice (Oryza sativa L.) genomic DNA by PCR. Their spatial and temporal expression patterns and expression potential in stable transgenic rice plants were examined with β-glucuronidase (GUS) used as a reporter gene. All the promoters showed the expected spatial expression within the endosperm. The GluA-1, GluA-2, and GluA-3 promoters directed GUS expression mainly in the outer portion (peripheral region) of the endosperm. The GluB-5 and GluC promoters directed GUS expression in the whole endosperm, with the latter expressed almost evenly throughout the whole endosperm, a feature different from that of other rice glutelin gene promoters. The GluB-3 promoter directed GUS expression solely in aleurone and subaleurone layers. Promoter activities examined during seed maturation showed that the GluC promoter had much higher activity than the other promoters. These promoters are ideal candidates for achieving gene expression for multiple purposes in monocot endosperm but avoid promoter homology-based gene silencing. The GluC promoter did not contain the endosperm specificity-determining motifs GCN4, AACA, and the prolamin-box, which suggests the existence of additional regulatory mechanism in determining endosperm specificity.
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影响因子：
5.1
作者：
BATTRAW, MJ;HALL, TC
通讯作者：
HALL, TC
DOI：
10.1073/pnas.0931425100
发表时间：
2003-05-13
影响因子：
11.1
作者：
Lin, L;Liu, YG;Li, BJ
通讯作者：
Li, BJ
影响因子：
5.1
作者：
Chen, Qi-Jun;Zhou, Hai-Meng;Wang, Xue-Chen
通讯作者：
Wang, Xue-Chen
影响因子：
7.4
作者：
Bhullar, S;Chakravarthy, S;Burma, PK
通讯作者：
Burma, PK
影响因子：
6.2
作者：
Choi, HW;Lemaux, PG;Cho, MJ
通讯作者：
Cho, MJ