MOLECULAR CHARACTERIZATION OF DROUGHT TOLER-ANCE IN NINE EGYPTIAN RICE GENOTYPES USING RAPD, SCoT AND SSR MARKERS
MOLECULAR CHARACTERIZATION OF DROUGHT TOLER-ANCE IN NINE EGYPTIAN RICE GENOTYPES USING RAPD, SCoT AND SSR MARKERS
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使用 RAPD、SCoT 和 SSR 标记对九种埃及水稻基因型的耐旱性进行分子表征
DOI：
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
M. Abo
中科院分区：
文献类型：
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作者：
A. A. Aboulila;O. Galal;M. Abo
使用三种不同类型的分子标记（RAPD、SCoT 和 SSR）评估了九种埃及水稻 (Oryza sativa L.) 基因型的 DNA 多态性。 RAPD引物产生68.70%的多态性，平均PIC值为0.24。 SCoT引物产生86.54%的多态性和0.28的PIC平均值。所有测试的 SSR 标记均产生扩增产物并生成 104 个等位基因（平均 6.5 个等位基因/标记），每个标记的 PIC 值范围为 0.20 至 0.49。 RM301 SSR 标记仅在四种干旱敏感水稻品种中产生特定等位基因，可以轻松区分敏感基因型和其他基因型。同时，RM20A 和 RM302 SSR 标记分别在中度品种（Sakha 102）和耐旱品种（Giza 178）中产生了一个阳性独特标记。这些标记可有效地用于水稻耐旱性的分子育种。另一方面，使用UPGMA方法的聚类分析将九种水稻基因型分为使用RAPD和SCoT标记的三组和使用SSR标记的四组。主坐标分析（PCoA）的结果与聚类分析的结果密切相关。育种者可以利用这些结果来开发耐旱水稻基因型和用于水稻改良的新育种方案。
Nine Egyptian rice ( Oryza sativa L.) genotypes were assessed for DNA polymorphism using three different types of molecular markers (RAPD, SCoT and SSR). RAPD primers produced 68.70% of polymorphism and an average PIC value of 0.24. SCoT primers generated 86.54% of polymorphism and 0.28 average value of PIC. All tested SSR markers yielded amplified products and generated 104 alleles (average 6.5 alleles/marker) with PIC values ranged from 0.20 to 0.49 per marker. RM301 SSR marker produced specific alleles only in the four drought sensitive rice varieties that could readily distinguished the sensitive genotypes from the others. While, RM20A and RM302 SSR markers produced one positive unique marker in the moderate (Sakha 102) and drought tolerant (Giza 178) varieties, respectively. These markers may be usefully exploited for molecular breeding of rice for drought tolerance. On the other hand, clustering analysis using UPGMA method classified the nine rice genotypes into three groups using RAPD and SCoT markers and four groups using SSR marker. The results of principal coordinate analysis (PCoA) were closely related with those of the clustering analysis. These results could be used by breeders to develop drought tolerant rice genotypes and new breeding protocols for rice improvement.