Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices
Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices
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DOI：
10.1073/pnas.0608897104
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发表时间：
2007-01-23
影响因子：
11.1
通讯作者：
Zhou, Z.
中科院分区：
文献类型：
--
作者：
Huang, H. -P.;Wang, S. -R.;Zhou, Z.
蓝斑（LC）含有一组紧凑的去甲肾上腺素能细胞体，投射到中枢神经系统的几乎所有部位。采用电流法和膜片钳技术相结合的方法，在LC神经元去极化后，检测到去甲肾上腺素（NA）的量子囊泡释放。脉冲去极化后，电流尖峰的平均潜伏期为1,870 ms，而谷氨酸介导的兴奋性突触后电流的潜伏期为1.6 ms。去极化诱导的电流尖峰的相当大一部分来自胞体。与谷氨酸介导的兴奋性突触后电流相反，NA分泌受到动作电位频率（0.5-50 Hz）的强烈调制。增强细胞活性后，LC的体树突状细胞NA释放产生了放电和NA释放的自抑制。我们的结论是，与经典的突触传递，量子NA释放LC胞体的特点是由一些不同的属性，包括长潜伏期和动作电位频率的高敏感性。
The locus coeruleus (LC) harbors a compact group of noradrenergic cell bodies projecting to virtually all parts of the central nervous system. By using combined measurements of amperometry and patch-clamp, quantal vesicle release of noradrenaline (NA) was detected as amperometric spikes, after depolarization of the LC neurons. After a pulse depolarization, the average latency of amperometric spikes was 1,870 ms, whereas the latency of glutamate-mediated excitatory postsynaptic currents was 1.6 ms. A substantial fraction of the depolarization-induced amperometric spikes originated from the somata. In contrast to glutamate-mediated excitatory postsynaptic currents, NA secretion was strongly modulated by the action potential frequency (0.5-50 Hz). Somatodendritic NA release from LC upon enhanced cell activity produced autoinhibition of firing and of NA release. We conclude that, in contrast to classic synaptic transmission, quantal NA release from LC somata is characterized by a number of distinct properties, including long latency and high sensitivity to action potential frequency.