Multilaboratory Survey To Evaluate Salmonella Prevalence in Diarrheic and Nondiarrheic Dogs and Cats in the United States between 2012 and 2014.
Multilaboratory Survey To Evaluate Salmonella Prevalence in Diarrheic and Nondiarrheic Dogs and Cats in the United States between 2012 and 2014.
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DOI：
10.1128/jcm.02137-16
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发表时间：
2017-05
影响因子：
9.4
通讯作者：
Okwumabua O
中科院分区：
文献类型：
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作者：
Reimschuessel R;Grabenstein M;Guag J;Nemser SM;Song K;Qiu J;Clothier KA;Byrne BA;Marks SL;Cadmus K;Pabilonia K;Sanchez S;Rajeev S;Ensley S;Frana TS;Jergens AE;Chappell KH;Thakur S;Byrum B;Cui J;Zhang Y;Erdman MM;Rankin SC;Daly R;Das S;Ruesch L;Lawhon SD;Zhang S;Baszler T;Diaz-Campos D;Hartmann F;Okwumabua O
11个实验室合作，以确定沙门氏菌的周期性流行在一群狗和猫在美国访问兽医诊所。在2012年1月至2014年4月期间，从36个州的11个地理分散的兽医检测实验室收集了粪便样本（2965份），并使用统一的方法进行了测试。研究中542只猫中沙门氏菌的总体流行率（3只）<1%。犬的患病率为2.5%（2422只犬中有60只）。只有55%的阳性犬出现腹泻；然而，在所有腹泻狗中，3.8%的狗呈阳性，而在非腹泻狗中，这一比例为1.8%。沙门氏菌阳性的狗更有可能食用生食（P = 0.01）、食用益生菌（P = 0.002）或服用抗生素（P = 0.01）。乡村犬也比城市犬（P = 0.002）或郊区犬（P = 0.001）更容易呈沙门氏菌阳性。在67株分离株中，鉴定出27种独特的血清型，其中3只犬存在两种血清型。66株菌株的药敏试验显示，仅有4株菌株对一种或多种抗生素耐药。利用脉冲场凝胶电泳和全基因组测序（WGS）对66株分离株进行了进一步的鉴定。序列数据与抗性表型数据比较良好，并提交给国家生物技术信息中心（NCBI）。这项研究表明，在过去的几十年里，沙门氏菌阳性的狗和猫的流行率总体下降，并确定食用生食是沙门氏菌感染的主要风险因素。值得注意的是，几乎一半的沙门氏菌阳性动物临床无腹泻。
Eleven laboratories collaborated to determine the periodic prevalence of Salmonella in a population of dogs and cats in the United States visiting veterinary clinics. Fecal samples (2,965) solicited from 11 geographically dispersed veterinary testing laboratories were collected in 36 states between January 2012 and April 2014 and tested using a harmonized method. The overall study prevalence of Salmonella in cats (3 of 542) was <1%. The prevalence in dogs (60 of 2,422) was 2.5%. Diarrhea was present in only 55% of positive dogs; however, 3.8% of the all diarrheic dogs were positive, compared with 1.8% of the nondiarrheic dogs. Salmonella-positive dogs were significantly more likely to have consumed raw food (P = 0.01), to have consumed probiotics (P = 0.002), or to have been given antibiotics (P = 0.01). Rural dogs were also more likely to be Salmonella positive than urban (P = 0.002) or suburban (P = 0.001) dogs. In the 67 isolates, 27 unique serovars were identified, with three dogs having two serovars present. Antimicrobial susceptibility testing of 66 isolates revealed that only four of the isolates were resistant to one or more antibiotics. Additional characterization of the 66 isolates was done using pulsed-field gel electrophoresis and whole-genome sequencing (WGS). Sequence data compared well to resistance phenotypic data and were submitted to the National Center for Biotechnology Information (NCBI). This study suggests an overall decline in prevalence of Salmonella-positive dogs and cats over the last decades and identifies consumption of raw food as a major risk factor for Salmonella infection. Of note is that almost half of the Salmonella-positive animals were clinically nondiarrheic.