Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish.
Mutations affecting cell fates and cellular rearrangements during gastrulation in zebrafish.
复制标题
DOI：
--
复制
发表时间：
1996-12
期刊：
影响因子：
4.6
通讯作者：
L. Solnica-Krezel;D. L. Stemple;E. Mountcastle-Shah;Z. Rangini;S. Neuhauss;J. Malicki;A. Schier;Didier Y. R. Stainier;F. Zwartkruis;S. Abdelilah;W. Driever
中科院分区：
文献类型：
--
作者：
L. Solnica-Krezel;D. L. Stemple;E. Mountcastle-Shah;Z. Rangini;S. Neuhauss;J. Malicki;A. Schier;Didier Y. R. Stainier;F. Zwartkruis;S. Abdelilah;W. Driever
发育生物学的主要挑战之一是了解形成脊椎动物胚胎的诱导和形态发生过程。在一个大规模的遗传筛选合子效应，胚胎致死突变斑马鱼，我们已经确定了25个突变，影响规范的细胞命运和/或细胞重排原肠胚形成。这些突变定义了至少14个互补组，其中4个对应于先前鉴定的基因。10个新位点的表型分析揭示了三组突变对原肠胚中细胞命运的不同影响。一组包括导致背侧中胚层命运缺陷并影响中枢神经系统模式的突变。第二组突变影响胚胎腹后结构的形成。我们认为，在这两个组中的突变确定必要的基因的形成，维持或功能的背侧组织者和腹侧信号通路，分别。第三组中的突变主要影响原肠胚形成期间的细胞重排，并且对胚胎中的细胞命运具有复杂的影响。这一组，并在一定程度上从前两组的突变，影响主要的形态发生过程，外包，收敛和延伸，和尾部形态发生。这些突变为理解脊椎动物原肠胚形成的遗传控制提供了一种方法。
One of the major challenges of developmental biology is understanding the inductive and morphogenetic processes that shape the vertebrate embryo. In a large-scale genetic screen for zygotic effect, embryonic lethal mutations in zebrafish we have identified 25 mutations that affect specification of cell fates and/or cellular rearrangements during gastrulation. These mutations define at least 14 complementation groups, four of which correspond to previously identified genes. Phenotypic analysis of the ten novel loci revealed three groups of mutations causing distinct effects on cell fates in the gastrula. One group comprises mutations that lead to deficiencies in dorsal mesodermal fates and affect central nervous system patterning. Mutations from the second group affect formation of ventroposterior embryonic structures. We suggest that mutations in these two groups identify genes necessary for the formation, maintenance or function of the dorsal organizer and the ventral signaling pathway, respectively. Mutations in the third group affect primarily cellular rearrangements during gastrulation and have complex effects on cell fates in the embryo. This group, and to some extent mutations from the first two groups, affect the major morphogenetic processes, epiboly, convergence and extension, and tail morphogenesis. These mutations provide an approach to understanding the genetic control of gastrulation in vertebrates.