Mesenchymal epimorphin is important for pancreatic duct morphogenesis
Mesenchymal epimorphin is important for pancreatic duct morphogenesis
复制标题
DOI：
10.1111/j.1440-169x.2006.00846.x
复制
发表时间：
2006-02-01
影响因子：
2.5
通讯作者：
Gittes, GK
中科院分区：
文献类型：
--
作者：
Tulachan, SS;Doi, R;Gittes, GK
上皮-间充质的相互作用对许多器官的正常发育至关重要，包括胰腺。在胰腺内，这些导管被认为含有干细胞/祖细胞，并可能导致胰腺导管癌。关于胚胎中胰管的形成机制，人们知之甚少。胰腺间充质含有大量的可溶性因子，有助于维持外分泌和内分泌结构的生长和分化。在此，我们报道了一种这样的形态调节间充质蛋白，表吗啡，在胰腺导管的增殖和分化过程中发挥着重要作用。我们发现，在发育早期，表吗啡在胰腺间充质中有表达，在发育后期，在胰管周围的间充质-上皮界面有表达。在体外胰管分化过程中，表吗啡的表达明显上调。类似地，体外胰管形成可被中和抗体所抑制，而重组表莫芬的加入部分挽救了胰管形成。综上所述，我们的研究证实了表吗啡在胰腺导管形态发生中的作用。
Epithelial-mesenchymal interactions are crucial for the proper development of many organs, including the pancreas. Within the pancreas, the ducts are thought to harbor stem/progenitor cells, and possibly to give rise to pancreatic ductal carcinoma. Little is known about the mechanism of formation of pancreatic ducts in the embryo. Pancreatic mesenchyme contains numerous soluble factors which help to sustain the growth and differentiation of exocrine and endocrine structures. Here, we report that one such morphoregulatory mesenchymal protein, epimorphin, plays an important role during pancreatic ductal proliferation and differentiation. We found that epimorphin is expressed in pancreatic mesenchyme during early stages of development, and at mesenchymal-epithelial interfaces surrounding the ducts at later stages. Strong upregulation of epimorphin expression was seen during in vitro pancreatic duct differentiation. Similarly, in vitro pancreatic duct formation was inhibited by a neutralizing antibody against epimorphin, whereas addition of recombinant epimorphin partially rescued duct formation. Together, our study demonstrates the role of epimorphin in pancreatic ductal morphogenesis.