Possible role of bacterial and viral infections in miscarriages
Possible role of bacterial and viral infections in miscarriages
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DOI：
10.1016/j.fertnstert.2003.08.020
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发表时间：
2004-03-01
影响因子：
6.7
通讯作者：
Grce, M
中科院分区：
文献类型：
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作者：
Matovina, M;Husnjak, K;Grce, M
目的：确定感染在流产中的作用。对流产材料的绒毛膜绒毛进行细胞遗传学评估，并分析是否存在沙眼衣原体、解脲支原体、人型支原体、人巨细胞病毒 (HCMV)、腺相关病毒 (AAV) 和人乳头瘤病毒 (HPV)。设计：回顾性研究。地点：大学医院和学术研究机构。主要结果指标：核型分析结果：108 份样本中，54 份（50%）核型正常，38 份（35%）样本核型异常，16 份（15%）样本核型未定。未检测到解脲支原体、人型分枝杆菌、HCMV 或 AAV-2 DNA，而在 1 个 (1%) 样本中检测到沙眼衣原体 DNA，在 8 个 (7%) 样本中检测到 HPV DNA。 HPV 阳性结果与核型状态没有显着相关性。结论：我们的研究结果不支持沙眼衣原体、解脲支原体、人型分枝杆菌、HCMV 或 AAV 感染在妊娠前三个月流产中的作用。然而，应进行进一步调查以确定 HPV 是否可能参与胎儿遗传异常的发生和流产。
Objective: To determine the role of infections in miscarriages. Chorionic villi from aborted material were subjected to cytogenetic evaluation and analyzed for the presence of Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, human cytomegalovirus (HCMV), adeno-associated virus (AAV), and human papillomaviruses (HPV).Design: Retrospective study.Setting: University hospital and academic research institution.Main Outcome Measure(s): Karyotyping and detection of bacterial and viral DNA by means of polymerase chain reaction (PCR) in placenta specimens.Result(s): In 54 (50%) of 108 samples the karyotype was normal, in 38 (35%) samples it was abnormal, and in 16 (15%) samples karyotype was undetermined. No U. urealyticum, M. hominis, HCMV, or AAV-2 DNA was detected, while C. trachomatis DNA was detected in one (1%) and HPV DNA in eight (7%) samples. No significant correlation of HPV-positive findings with karyotype status was established.Conclusion(s): Our findings do not support a role of C. trachomatis, U. urealyticum, M. hominis, HCMV, or AAV infections in miscarriages during the first trimester of pregnancy. However, further investigation should be made to determine a possible involvement of HPVs in the development of genetic abnormalities of the fetus and in miscarriages.