The composition of liquid atmospheric pressure matrix-assisted laser desorption/ionization matrices and its effect on ionization in mass spectrometry
The composition of liquid atmospheric pressure matrix-assisted laser desorption/ionization matrices and its effect on ionization in mass spectrometry
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DOI：
10.1016/j.aca.2018.01.070
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发表时间：
2018-07-12
影响因子：
6.2
通讯作者：
Cramer, Rainer
中科院分区：
文献类型：
--
作者：
Ryumin, Pavel;Cramer, Rainer
新的液体大气压（AP）基质辅助激光解吸/电离（MALDI）矩阵，主要产生多电荷离子已开发和评价其性能的肽和蛋白质的质谱分析（MS）。这些基质中的发色团和粘性支持液均针对最高MS信号强度、S/N值和最大电荷状态进行了优化。采用轻二醇作为基质支持液（例如乙二醇和丙二醇）在蛋白质和肽分析中实现了最佳性能。研究发色团的影响，发现2，5_二羟基苯甲酸导致用于分析小肽的更高的分析物离子信号强度;然而，检测不到更大的分子（>17 kDa）。对于较大的分子，样品制备的基础上α-氰基-4-羟基肉桂酸作为发色团的开发和多重质子化的分析物的电荷状态超过50检测。因此，第一次有可能用MALDI MS蛋白质检测到类似于80 kDa的大蛋白质，具有大量电荷状态，即m/z值低于2000。各种基质支持液体的系统研究已经揭示了激光阈值能量和液体MALDI样品的表面张力之间的线性依赖性。(C)2018爱思唯尔B. V.保留所有权利。
New liquid atmospheric pressure (AP) matrix-assisted laser desorption/ionization (MALDI) matrices that produce predominantly multiply charged ions have been developed and evaluated with respect to their performance for peptide and protein analysis by mass spectrometry (MS). Both the chromophore and the viscous support liquid in these matrices were optimized for highest MS signal intensity, S/N values and maximum charge state. The best performance in both protein and peptide analysis was achieved employing light diols as matrix support liquids (e.g. ethylene glycol and propylene glycol). Investigating the influence of the chromophore, it was found that 2,5-dihydroxybenzoic acid resulted in a higher analyte ion signal intensity for the analysis of small peptides; however, larger molecules (>17 kDa) were undetectable. For larger molecules, a sample preparation based on alpha-cyano-4-hydroxycinnammic acid as the chromophore was developed and multiply protonated analytes with charge states of more than 50 were detected. Thus, for the first time it was possible to detect with MALDI MS proteins as large as similar to 80 kDa with a high number of charge states, i.e. m/z values below 2000. Systematic investigations of various matrix support liquids have revealed a linear dependency between laser threshold energy and surface tension of the liquid MALDI sample. (C) 2018 Elsevier B.V. All rights reserved.