Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.
Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers.
复制标题
DOI：
10.1039/c9sm01945f
复制
发表时间：
2020-03
期刊：
影响因子：
3.4
通讯作者：
Tao Sun;Yibing Yao;Qing Shi;Huaping Wang;P. Dario;Junzhong Sun;Qiang Huang;T. Fukuda
中科院分区：
文献类型：
--
作者：
Tao Sun;Yibing Yao;Qing Shi;Huaping Wang;P. Dario;Junzhong Sun;Qiang Huang;T. Fukuda
与高浓度相比，低浓度的明胶甲基丙烯酸酯（GelMA）微纤维更有利于细胞活性。然而，由于低浓度GelMA微纤维的机械性能差，将其用作高阶细胞组装的构建块仍然具有挑战性。在这里，我们报告了一种新的基于模板的方法来解决这个问题。通过微流控纺丝方法合成含有磁性纳米颗粒的GelMA微纤维（5%，w/v）。构建了一个9 × 9的微柱阵列，在磁性基底周围形成8 × 8的微间隙，作为磁性模板。在DMEM溶液中，磁吸引力促进了根据模板的微纤维的有效排列，具有微米组装精度，在去除所有微柱后产生微网格状构造（microGC）。MicroGC显示出有效地支持表面接种或包封的细胞的活性，并且被灵活地构建有各种有组织的空间模式。由于组装微纤维的低机械性能要求和易于实施的操作，所提出的方法提供了一个通用的路径，为各种微流体纺微纤维的组装。此外，所得到的三维微网格状细胞结构与组织的时空组成提供了一个方便的平台，用于组织工程的研究。
Low concentrations of gelatin methacrylate (GelMA) microfibers are more favorable for cellular activity compared with high concentrations. However, applying low-concentration GelMA microfibers as building blocks for higher-order cellular assembly remains challenging owing to their poor mechanical properties. Herein, we report a new template-based method to solve this problem. GelMA microfibers (5%, w/v) containing magnetic nanoparticles were synthesized by a microfluidic spinning method. A 9 × 9 micropillar array surrounded by a magnetic substrate was constructed to form 8 × 8 microgaps arranged in a crisscross pattern as a magnetic template. In DMEM solution, magnetic attraction facilitated efficient arrangement of the microfibers according to the template with micron assembly accuracy, with a microgrid-like construct (microGC) generated after removing all micropillars. MicroGCs were shown to effectively support the activities of surface seeded or encapsulated cells and be flexibly constructed with various organized spatial patterns. Owing to the low mechanical property requirements of assembled microfibers and the easy-to-implement operation, the proposed method provides a versatile pathway for the assembly of various microfluidic spun microfibers. Furthermore, the resulting 3D microgrid-like cellular constructs with organized spatiotemporal composition offer a convenient platform for the study of tissue engineering.