The scallop IGF2 mRNA-binding protein gene PyIMP and association of a synonymous mutation with growth traits
The scallop IGF2 mRNA-binding protein gene PyIMP and association of a synonymous mutation with growth traits
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扇贝IGF2 mRNA结合蛋白基因PyIMP及其同义突变与生长性状的关联
DOI：
10.1266/ggs.17-00028
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发表时间：
2018-06-01
影响因子：
1.1
通讯作者：
Bao, Zhenmin
中科院分区：
文献类型：
--
作者：
Ning, Xianhui;Feng, Liying;Bao, Zhenmin
胰岛素样生长因子2 mRNA结合蛋白（IMP）在其靶RNA的定位、稳定性和翻译控制中起作用。本研究从虾夷扇贝（Patinopecten yessoensis）中克隆了一个IMP基因（PyIMP）。PyIMP基因全长22，875 bp，由17个外显子和16个内含子组成。其cDNA全长为3,293 bp，开放阅读框为1,776 bp，编码592个氨基酸。PyIMP具有IMP的典型特征，即两个RNA识别基序和四个hnRNP K同源结构域。实时荧光定量RT-PCR分析表明，PyIMP在扇贝中普遍表达，在成体性腺、原肠胚和担轮幼虫中表达量较高。在该基因中发现了一个与虾夷扇贝生长性状显著相关的同义突变SNP，c.852A>G。在壳长、壳高、体重、软组织重和横纹肌重等性状上，AA基因型的性状值显著高于GG基因型（P < 0.05）。同时，PyIMP在AA型扇贝中的表达量显著高于GG型扇贝，表明PyIMP对扇贝生长有促进作用。PyIMP是第一个在软体动物中发现的mRNA结合蛋白基因，其SNP c.852A>G将有助于更好地了解mRNA结合蛋白在贝类中的作用，并为扇贝育种提供参考。
Insulin-like growth factor 2 mRNA-binding proteins (IMPs) function in localization, stability and translational control of their target RNAs. In this study, we identified an IMP gene (PyIMP) from Yesso scallop, Patinopecten yessoensis. The complete DNA sequence of PyIMP was 22,875 bp, consisting of seventeen exons and sixteen introns. The full-length cDNA sequence was 3,293 bp, with an open reading frame of 1,776 bp, encoding 592 amino acids. PyIMP exhibited characters typical of IMPs, namely two RNA recognition motifs and four hnRNP K homology domains. Real-time quantitative reverse transcription PCR analysis indicated that PyIMP was universally expressed, with higher expression levels in the gonad of adult scallops, and in gastrulae and trochophore larvae at developmental stages. A synonymous mutation SNP, c.852A>G, which showed significant associations with growth traits of Yesso scallop, was identified in this gene. Scallops with the AA genotype at this locus had significantly higher trait values than those with the GG genotype for shell length, shell height, body weight, soft tissue weight and striated muscle weight (P < 0.05). Meanwhile, the expression of PyIMP in AA type scallops was significantly higher than that in the GG type, implying a positive effect of PyIMP on scallop growth. PyIMP represents the first mRNA-binding protein gene characterized in mollusks, and SNP c.852A>G will be useful for a better understanding of the role of mRNA-binding proteins in bivalves and for scallop breeding.