Searching for first-degree familial relationships in California's offender DNA database: Validation of a likelihood ratio-based approach
Searching for first-degree familial relationships in California's offender DNA database: Validation of a likelihood ratio-based approach
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DOI：
10.1016/j.fsigen.2010.10.010
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发表时间：
2011-11-01
影响因子：
3.1
通讯作者：
Buoncristiani, Martin R.
中科院分区：
文献类型：
--
作者：
Myers, Steven P.;Timken, Mark D.;Buoncristiani, Martin R.
通过比较常染色体短串联重复序列（STR）特征与加州相似的1，000，000个特征的国家DNA索引系统（SDIS）数据库，进行验证研究以测量使用基于似然比的方法搜索可能的一级家族关系（全同胞和亲子）的有效性。测试搜索使用了100个人工测试家族的常染色体STR和Y-STR图谱。当测试样本和数据库中的一级亲属在15个Identifiler（R）（Applied Biosystems（R），Foster City，CA）STR基因座处进行表征时，搜索程序包括96%的父亲和72%的全同胞。当相对图谱仅限于13个联合DNA索引系统（CODIS）核心位点时，搜索程序包括93%的父亲和61%的全同胞。这些结果，结合使用三个真实的家庭的功能测试，支持该工具的有效性。基于这些结果，验证的方法被实施为一个关键的，务实的和明显实用的组成部分，加州司法部的家庭搜索程序。通过这一过程创造的一个调查线索最近导致了洛杉矶冷酷沉睡者系列谋杀案的逮捕。出版社：Elsevier爱尔兰Ltd.
A validation study was performed to measure the effectiveness of using a likelihood ratio-based approach to search for possible first-degree familial relationships (full-sibling and parent-child) by comparing an evidence autosomal short tandem repeat (STR) profile to California's similar to 1,000,000-profile State DNA Index System (SDIS) database. Test searches used autosomal STR and Y-STR profiles generated for 100 artificial test families. When the test sample and the first-degree relative in the database were characterized at the 15 Identifiler (R) (Applied Biosystems (R), Foster City, CA) STR loci, the search procedure included 96% of the fathers and 72% of the full-siblings. When the relative profile was limited to the 13 Combined DNA Index System (CODIS) core loci, the search procedure included 93% of the fathers and 61% of the full-siblings. These results, combined with those of functional tests using three real families, support the effectiveness of this tool. Based upon these results, the validated approach was implemented as a key, pragmatic and demonstrably practical component of the California Department of Justice's Familial Search Program. An investigative lead created through this process recently led to an arrest in the Los Angeles Grim Sleeper serial murders. Published by Elsevier Ireland Ltd.