Identical origin of adrenal cortex and gonad revealed by expression profiles of Ad4BP/SF-1
Identical origin of adrenal cortex and gonad revealed by expression profiles of Ad4BP/SF-1
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DOI：
10.1046/j.1365-2443.1996.00254.x
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发表时间：
1996-07-01
期刊：
影响因子：
2.1
通讯作者：
Morohashi, K
中科院分区：
文献类型：
--
作者：
Hatano, O;Takakusu, A;Morohashi, K
背景：Ad 4 BP/SF-1最初被鉴定为类固醇生成的组织特异性转录因子。最近的基因破坏与哺乳动物Ftz-F1基因编码的Ad 4 BP/SF-1的研究清楚地揭示了肾上腺和性腺分化的因子的基本功能。结果：在这项研究中，我们研究了这些组织的早期发展，使用Ad 4 BP/SF-1作为标记。在妊娠后11.5天（d.p.c.）的大鼠胎仔中，首先在从背主动脉延伸到原始泌尿生殖嵴的背体腔上皮的对称线上观察到称为肾上腺生殖原基的细胞群。从12.5 d.p.c.，肾上腺生殖原基的喙侧的一半开始分离成两个不同的细胞群。从原始生殖细胞的分布来看，背腹侧的细胞群是肾上腺皮质的原基，而体腔上皮侧的细胞群是性腺的原基。在13.5 d.p.c.，结论：这些观察清楚地确定了一种新的肾上腺-生殖器原基，肾上腺皮质和性腺都起源于此。一项检测肾上腺和性腺特异性mRNA的RT-PCR研究支持了上述观察结果。
Background: Ad4BP/SF-1 was originally identified as a steroidogenic tissue-specific transcription factor. Recent gene disruption studies with the mammalian Ftz-F1 gene encoding Ad4BP/SF-1 clearly revealed the essential function of the factor for adrenal and gonadal differentiation.Results: In this study, we examined the early development of these tissues using Ad4BP/SF-1 as the marker. In rat foetuses of 11.5 days post-coitum (d.p.c.), a cell population designated adrenogenital primordium was firstly observed on symmetrical lines extending from the dorsal aorta to the dorsal coelomic epithelia of the primitive urogenital ridges. From 12.5 d.p.c., the rostral half of the adreno-genital primordium started to separate into two distinct cell populations. Judging from the distribution of primordial germ cells, the cell population on the dorsal aortal side is a primordium for the adrenal cortex whereas that on the coelomic epithelial side is for the gonads. At 13.5 d.p.c., these two primordia have separated completely.Conclusion: These observations clearly identified a novel adreno-genital primordium from which both the adrenal cortex and the gonads originate. An RT-PCR study conducted to detect adrenal- and gonad-specific mRNAs supported the above observations.