Complete sequence and analysis of the ovine herpesvirus 2 genome
Complete sequence and analysis of the ovine herpesvirus 2 genome
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DOI：
10.1099/vir.0.82284-0
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发表时间：
2007-01-01
影响因子：
3.8
通讯作者：
Stewart, James P.
中科院分区：
文献类型：
--
作者：
Hart, Jane;Ackermann, Mathias;Stewart, James P.
羊疱疹病毒2 （OvHV-2）在全世界的绵羊种群中流行，并在牛、野牛和鹿中引起恶性卡他性热（MCF），这是一种淋巴细胞增生性疾病。OvHV-2属于γ疱疹病毒亚家族，与阿尔celaphine herpesvirus 1 （AIHV-1）密切相关。本文报道了来自受感染奶牛（BJ1 035）的淋巴母细胞样细胞系的OvHV-2全基因组的克隆、测序和分析。基因组的独特部分由130930 bp组成，平均G+C含量为52 mol%。独特的DNA两侧有多个末端重复序列，长度为4205 bp，平均G+C含量为72 mol%。分析发现73个开放阅读框（orf），其中大多数（62个）显示与其他γ疱疹病毒基因同源。9个orf的另一个子集仅与相关的AIHV-1共享。三个orf是OvHV-2完全独特的，包括细胞白细胞介素-10的剪接同源物，保留细胞基因的外显子结构。OvHV-2的序列是研究MCF发病机制和治疗的关键第一步。
Ovine herpesvirus 2 (OvHV-2) is endemic in sheep populations worldwide and causes malignant catarrhal fever (MCF), a lymphoproliferative disease, in cattle, bison and deer. OvHV-2 has been placed in the gammaherpesvirus subfamily and is related closely to Alcelaphine herpesvirus 1 (AIHV-1). Here, the cloning, sequencing and analysis of the complete OvHV-2 genome derived from a lymphoblastoid cell line from an affected cow (BJ1 035) are reported. The unique portion of the genome consists of 130930 bp, with a mean G+C content of 52 mol%. The unique DNA is flanked by multiple copies of terminal repeat elements 4205 bp in length, with a mean G+C content of 72 mol%. Analysis revealed 73 open reading frames (ORFs), the majority (62) of which showed homology to other gammaherpesvirus genes. A further subset of nine ORFs is shared with only the related AIHV-1. Three ORFs are entirely unique to OvHV-2, including a spliced homologue of cellular interleukin-10 that retains the exon structure of the cellular gene. The sequence of OvHV-2 is a critical first step in the study of the pathogenesis and treatment of MCF.