Acute neuroestrogen blockade attenuates song-induced immediate early gene expression in auditory regions of male and female zebra finches.
Acute neuroestrogen blockade attenuates song-induced immediate early gene expression in auditory regions of male and female zebra finches.
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急性神经雌激素阻断减弱了雄性和雌性斑胸草雀听觉区域鸣叫诱导的早期基因表达。
DOI：
10.1007/s00359-019-01382-w
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Remage-Healey,Luke
中科院分区：
文献类型：
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作者：
Krentzel,AmandaA;Ikeda,MaayaZ;Oliver,TessaJ;Koroveshi,Era;Remage-Healey,Luke
神经源性雌激素是由芳香酶合成的，通过膜受体调节神经生理。在许多系统中，长期的激素治疗可以改变感觉诱发的神经元激活。然而，人们对急性神经雌激素产生的意义知之甚少。雌雄斑纹雀都能在听觉皮质快速合成雌激素，但目前还不清楚这是如何调节神经细胞信号的。我们研究了急性雌激素合成阻断是否减弱了听觉诱导的早期生长反应1(Egr-1)在两性听觉皮质中的表达。同时还检测了SONG刺激诱导的cAMP反应元件结合蛋白磷酸化(PCREB)和急性雌激素合成。我们在SONG暴露前使用芳香酶抑制剂Fadrozole，并测量了几个听觉区域的Egr-1。Fadrozole对听觉皮质中Egr-1的减弱作用在男性比女性更大。在高声带中心(HVC)区，女性芳香酶细胞的表达和聚集程度明显高于男性。Fadrozole治疗后，听觉诱导的Egr-1表达显示出很大的性别差异。我们没有观察到歌曲呈现或芳香酶阻断后pCREB表达的变化。这些发现与以下假设一致，即急性神经雌激素合成可以驱动几个皮质听觉区域的下游转录反应，并且这种机制在男性中更为突出。
Neuron-derived estrogens are synthesized by aromatase and act through membrane receptors to modulate neuronal physiology. In many systems, long-lasting hormone treatments can alter sensory-evoked neuronal activation. However, the significance of acute neuroestrogen production is less understood. Both sexes of zebra finches can synthesize estrogens rapidly in the auditory cortex, yet it is unclear how this modulates neuronal cell signaling. We examined whether acute estrogen synthesis blockade attenuates auditory-induced expression of early growth response 1 (Egr-1) in the auditory cortex of both sexes. cAMP response element-binding protein phosphorylation (pCREB) induction by song stimuli and acute estrogen synthesis was also examined. We administered the aromatase inhibitor fadrozole prior to song exposure and measured Egr-1 across several auditory regions. Fadrozole attenuated Egr-1 in the auditory cortex greater in males than females. Females had greater expression and clustering of aromatase cells than males in high vocal center (HVC) shelf. Auditory-induced Egr-1 expression exhibited a large sex difference following fadrozole treatment. We did not observe changes in pCREB expression with song presentation or aromatase blockade. These findings are consistent with the hypothesis that acute neuroestrogen synthesis can drive downstream transcriptional responses in several cortical auditory regions, and that this mechanism is more prominent in males.