Combination of simvastatin, calcium silicate/gypsum, and gelatin and bone regeneration in rabbit calvarial defects.
Combination of simvastatin, calcium silicate/gypsum, and gelatin and bone regeneration in rabbit calvarial defects.
复制标题
辛伐他汀、硅酸钙/石膏和明胶的组合与兔颅骨缺损的骨再生
DOI：
10.1038/srep23422
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发表时间：
2016-03-21
影响因子：
4.6
通讯作者：
Yang G
中科院分区：
文献类型：
--
作者：
Zhang J;Wang H;Shi J;Wang Y;Lai K;Yang X;Chen X;Yang G
本研究旨在确定辛伐他汀与硅酸钙/石膏和明胶(CS-Gel)联合使用是否能促进骨再生。用场发射扫描电子显微镜(FSEM)对其表面形貌进行了表征。通过监测复合材料在磷酸盐缓冲盐水(PBS)中的重量变化来评价其在体外的降解情况。采用高效液相色谱(HPLC法)评价药物释放。通过细胞毒性试验评价复合材料的生物相容性。在兔颅骨上造成4个5 mm直径的骨缺损。3个部位分别填充CS-Gel、0.5 mg辛伐他汀CS-Gel(SIM-0.5)和1.0 mg辛伐他汀CS-Gel(SIM-1)，第4个为空白对照组。分别于术后4周和12周行微计算机断层扫描(Micro-CT)和组织学检查。复合材料均为三维结构，浸泡4周后残留物接近80%。首日释药呈爆炸性，随后释药速率保持稳定。该复合材料不会产生任何细胞毒性。体内实验结果表明，辛伐他汀壳聚糖凝胶组的新骨形成及BMP-2、OC和I型胶原的表达均有改善。结论辛伐他汀CS凝胶具有促进骨再生的作用。
The present study was performed to determine whether simvastatin improves bone regeneration when combined with calcium silicate/gypsum and gelatin (CS-GEL). The surface morphology was determined using field-emission scanning electron microscopy (FSEM). Degradationin vitrowas evaluated by monitoring the weight change of the composites soaked in phosphate buffered saline (PBS). Drug release was evaluated using high-performance liquid chromatography (HPLC). Cytotoxicity testing was performed to assess the biocompatibility of composites. Four 5 mm-diameter bone defects were created in rabbit calvaria. Three sites were filled with CS-GEL, 0.5 mg simvastatin-loaded CS-GEL (SIM-0.5) and 1.0 mg simvastatin-loaded CS-GEL (SIM-1.0), respectively, and the fourth was left empty as the control group. Micro-computed tomography (micro-CT) and histological analysis were carried out at 4 and 12 weeks postoperatively. The composites all exhibited three-dimensional structures and showed the residue with nearly 80% after 4 weeks of immersion. Drug release was explosive on the first day and then the release rate remained stable. The composites did not induce any cytotoxicity. The resultsin vivodemonstrated that the new bone formation and the expressions of BMP-2, OC and type I collagen were improved in the simvastatin-loaded CS-GEL group. It was concluded that the simvastatin-loaded CS-GEL may improve bone regeneration.