Genomics of Black American colon cancer disparities: An RNA sequencing (RNA-Seq) study from an academic, tertiary referral center.
Genomics of Black American colon cancer disparities: An RNA sequencing (RNA-Seq) study from an academic, tertiary referral center.
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DOI：
10.1016/j.surg.2021.03.031
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发表时间：
2021-10
期刊：
影响因子：
3.8
通讯作者：
Agarwal S
中科院分区：
文献类型：
--
作者：
Haskins IN;Wang BD;Bernot JP;Cauley E;Horvath A;Marks JH;Lee NH;Agarwal S
与非西班牙裔白色美国人（NHWA）相比，美国黑人（BA）的结直肠癌发病率和死亡率更高。即使控制了这两个人群之间的社会人口统计学差异，BA仍然不成比例地受到结直肠癌的影响。我们研究的目的是确定BA和NHWA结肠癌标本之间基因表达的差异是否有助于解释这两个人群之间发病率和死亡率的差异。确定了在同一个机构接受I、II或III期结肠癌结肠切除术的BA和NHWA。BA和NHWA患者的年龄、性别和结肠癌分期相匹配，以最大限度地减少混杂变量的风险。在结肠切除时获得组织样本，并使用RNA测序（RNA-Seq）进行分析，以确定两组之间的基因表达和生物学过程是否存在差异。共分析了17例结肠癌标本; 8例（47.1%）患者为BA。与NHWA结肠癌标本相比，总共456个基因被鉴定为在BA中表达不同（即上调或下调）。此外，在我们对BA和NHWA结肠癌标本的比较中，发现500种不同的遗传途径被差异表达基因显著过度代表，其中大多数在炎症和免疫细胞功能中起作用。BA和NHWA在基因表达和遗传途径上存在显著差异。需要额外的多机构和基于注册的研究来验证我们的研究结果，并进一步阐明这些差异对这两个患者人群中结肠癌总体发病率和死亡率的贡献。结肠癌的发病率和死亡率在美国黑人（BA）和非西班牙裔白色美国人（NHWA）之间差异显著。这项研究使用RNA-Seq鉴定了BA和NHWA结肠癌标本之间456个基因和500个遗传通路的表达差异，这可能有助于解释这两个人群之间结肠癌进展的差异。
Black Americans (BA) have a higher incidence and mortality rate from colorectal cancer compared to their Non-Hispanic White American (NHWA) counterparts. Even when controlling for sociodemographic differences between these two populations, BA remain disproportionately affected by colorectal cancer. The purpose of our study was to determine if differences in gene expression between BA and NHWA colon cancer specimens could help explain differences in the incidence and mortality rate between these two populations. BA and NHWA undergoing colon resection for stage I, II, or III colon cancer at a single institution were identified. BA and NHWA patients were matched for age, gender, and colon cancer stage to minimize the risk of confounding variables. Tissue samples were obtained at the time of colon resection and were analyzed using RNA sequencing (RNA-Seq) to determine if there were differences in the expression of genes and biologic processes between the two groups. A total of 17 colon cancer specimens were analyzed; 8 (47.1%) patients were BA. A total of 456 genes were identified as being expressed differently (i.e. up- or down-regulated) in BA compared to NHWA colon cancer specimens. Moreover, 500 different genetic pathways were noted to be significantly over-represented with differentially expressed genes in our comparison of BA and NHWA colon cancer specimens, the majority of which plays a role in inflammation and immune cell function. Significant differences in gene expression and genetic pathways exist between BA and NHWA. Additional and multi-institutional and registry-based studies are needed to validate our findings and to further elucidate the contribution that these differences have to the overall incidence and mortality rate from colon cancer in these two patient populations. The incidence and mortality rate from colon cancer varies significantly between Black Americans (BA) and Non-Hispanic White Americans (NHWA). This study used RNA-Seq to identify difference in expression of 456 genes and 500 genetic pathways between BA and NHWA colon cancer specimens, which may help to explain differences in the progression of colon cancer between these two populations.
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影响因子：
4.6
作者：
Kosti I;Jain N;Aran D;Butte AJ;Sirota M
通讯作者：
Sirota M
影响因子：
29.4
作者：
Lai Y;Wang C;Civan JM;Palazzo JP;Ye Z;Hyslop T;Lin J;Myers RE;Li B;Jiang B;Sama A;Xing J;Yang H
通讯作者：
Yang H
影响因子：
1.9
作者：
Esnaola, Nestor F.;Ford, Marvella E.
通讯作者：
Ford, Marvella E.
影响因子：
14.9
作者：
McCarthy DJ;Chen Y;Smyth GK
通讯作者：
Smyth GK
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y