Microorganisms in vortices: a microfluidic setup
Microorganisms in vortices: a microfluidic setup
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DOI：
10.4319/lom.2006.4.392
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发表时间：
2006-11-01
影响因子：
2.7
通讯作者：
Stocker, Roman
中科院分区：
文献类型：
--
作者：
Marcos;Stocker, Roman
提出了一种利用专门为研究游泳微生物的响应而设计的微流体装置来产生微尺度漩涡的方法。在主流剪切应力作用下，微通道侧腔内产生稳定的小尺度涡流。研究发现，涡旋的产生取决于空腔的几何形状，特别是它的深度、纵横比和开口长度。利用视频显微镜，跟踪在不同强度的漩涡中游动的单个细菌和藻类的位置和方向。细长的细胞与强涡中的流线排列成一条直线。该实验系统提供了有关微生物与其流体动力环境之间相互作用的定量单细胞信息。
A method is presented to create microscale vortices using a microfluidic setup specifically designed to investigate the response of swimming microorganisms. Stable, small-scale vortices were generated in the side cavity of a microchannel by the shear stress in the main flow. The generation of a vortex was found to depend on the cavity's geometry, in particular its depth, aspect ratio, and opening length. Using video-microscopy, the position and orientation of individual bacteria and algae swimming in vortices of various strengths were tracked. Elongated cells were found to align with the streamlines in a strong vortex. The experimental system provides quantitative single-cell information on the interaction between microorganisms and their fluid dynamical environment.