Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells.
Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells.
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DOI：
10.2217/nnm.15.35
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Sitharaman B
中科院分区：
文献类型：
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作者：
Rashkow JT;Talukdar Y;Lalwani G;Sitharaman B
本研究调查了二硫化钨纳米管（WSNTs）和二硫化钼纳米片（MSNPs）对成纤维细胞（NIH-3 T3）和间充质干细胞（MSC）的影响，以确定潜在生物医学应用的安全剂量。在6、12或24小时评估MSNP和WSNTs（5-300 μg/ml）对NIH-3 T3和MSC的细胞毒性。在处理24小时后评估MSC向脂肪细胞和成骨细胞的分化。只有用MSNPs处理的NIH-3 T3细胞显示出细胞毒性的剂量或时间依赖性增加。与未处理的对照组相比，处理组中的MSC的分化标志物不受影响。浓度低于50 μg/ml的MSNP和WSNTs对于治疗成纤维细胞或MSC长达24小时是潜在安全的。
This study investigates the effects of tungsten disulfide nanotubes (WSNTs) and molybdenum disulfide nanoplatelets (MSNPs) on fibroblasts (NIH-3T3) and mesenchymal stem cells (MSCs) to determine safe dosages for potential biomedical applications. Cytotoxicity of MSNPs and WSNTs (5–300 µg/ml) on NIH-3T3 and MSCs was assessed at 6, 12 or 24 h. MSC differentiation to adipocytes and osteoblasts was assessed following treatment for 24 h. Only NIH-3T3 cells treated with MSNPs showed dose or time dependent increase in cytotoxicity. Differentiation markers of MSCs in treated groups were unaffected compared with untreated controls. MSNPs and WSNTs at concentrations less than 50 µg/ml are potentially safe for treatment of fibroblasts or MSCs for up to 24 h.