Dual origin of spinal oligodendrocyte progenitors and evidence for the cooperative role of Olig2 and Nkx2.2 in the control of oligodendrocyte differentiation.
Dual origin of spinal oligodendrocyte progenitors and evidence for the cooperative role of Olig2 and Nkx2.2 in the control of oligodendrocyte differentiation.
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发表时间：
2002-02
期刊：
影响因子：
4.6
通讯作者：
H. Fu;Y. Qi;Min Tan;Jun Cai;H. Takebayashi;M. Nakafuku;W. Richardson;M. Qiu
中科院分区：
文献类型：
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作者：
H. Fu;Y. Qi;Min Tan;Jun Cai;H. Takebayashi;M. Nakafuku;W. Richardson;M. Qiu
在本研究中，我们通过比较少突胶质细胞发生之前和期间胚胎鸡和小鼠脊髓中 Olig2 和 Nkx2.2 的表达，研究了 Olig2+ 和 Nkx2.2+ 少突胶质细胞祖细胞 (OLP) 的关系。在神经发生阶段，Olig2 和 Nkx2.2 在腹侧神经上皮的相邻非重叠域中表达。在少突胶质细胞发生阶段，这两个域产生不同的 OLP 群体。 Olig2+ 运动神经元前体结构域 (pMN) 产生 Olig2+/Pdgfra+ OLP，而 Nkx2.2+ p3 结构域产生 Nkx2.2+ OLP。尽管起源不同，但这两个 OLP 群体最终似乎在同一细胞中共表达 Olig2 和 Nkx2.2。然而，Olig2+/Pdgfra+ OLP 获得 Nkx2.2 表达的时间存在物种差异。 OLPs中Nkx2.2和Olig2的共表达与正常胚胎和PDGFA(-/-)胚胎中的髓磷脂基因表达密切相关，表明这些转录因子在控制少突胶质细胞分化中的协同作用。为了支持这一建议，反义寡核苷酸对培养物中这两种转录因子表达的抑制对 OLP 分化和蛋白脂质蛋白 (PLP) 基因表达具有附加抑制作用。
In this study, we have investigated the relationship of Olig2+ and Nkx2.2+ oligodendrocyte progenitors (OLPs) by comparing the expression of Olig2 and Nkx2.2 in embryonic chicken and mouse spinal cords before and during the stages of oligodendrogenesis. At the stages of neurogenesis, Olig2 and Nkx2.2 are expressed in adjacent non-overlapping domains of ventral neuroepithelium. During oligodendrogenesis stages, these two domains generate distinct populations of OLPs. From the Olig2+ motoneuron precursor domain (pMN) arise the Olig2+/Pdgfra+ OLPs, whereas the Nkx2.2+ p3 domain give rise to Nkx2.2+ OLPs. Despite their distinct origins, both populations of OLPs eventually appear to co-express Olig2 and Nkx2.2 in the same cells. However, there is a species difference in the timing of acquiring Nkx2.2 expression by the Olig2+/Pdgfra+ OLPs. The co-expression of Nkx2.2 and Olig2 in OLPs is tightly associated with myelin gene expression in the normal and PDGFA(-/-) embryos, suggesting a cooperative role of these transcription factors in the control of oligodendrocyte differentiation. In support of this suggestion, inhibition of expression of these two transcription factors in culture by antisense oligonucleotides has an additive inhibitory effect on OLP differentiation and proteolipid protein (PLP) gene expression.