Ex vivo measurement of tissue distribution of a nitroxide radical after intravenous injection and its in vivo imaging using a rapid scan ESR-CT system
Ex vivo measurement of tissue distribution of a nitroxide radical after intravenous injection and its in vivo imaging using a rapid scan ESR-CT system
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DOI：
10.1016/s0730-725x(99)00122-8
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发表时间：
2000-02-01
影响因子：
2.5
通讯作者：
Takahashi, T
中科院分区：
文献类型：
--
作者：
Togashi, H;Matsuo, T;Takahashi, T
为了确定 3-氨基甲酰基-2,2,5,5-四甲基吡咯烷-1-氧基（氨基甲酰基-PROXYL）在活体动物中进行 ESR-CT 成像的有效性，我们研究了静脉注射后氨基甲酰基-PROXYL 的组织分布。注射。注射氨基甲酰PROXYL后10分钟，其在肝、脾、肾和血浆中的浓度高于小肠和胃中的浓度。然而，器官间浓度差异并不显着。我们选择肝脏作为代表器官，并在用盐水原位灌注冲洗掉所有血液后尝试测量其中氨基甲酰-PROXYL的浓度。完全血液冲洗后肝脏匀浆的ESR谱显示氨基甲酰-PROXYL的浓度显着降低。因此，此时，氨基甲酰基-PROXYL主要分布在血浆中和/或松散地附着于细胞表面。我们在40秒的测量时间内获得了小鼠腹部的高质量ESR-CT图像，发现肝脏和肾脏中出现了氨基甲酰-PROXYL的高强度区域，表明血液循环丰富。尽管氨基甲酰-PROXYL 的器官特异性较弱，但我们认为氨基甲酰-PROXYL 的 ESR-CT 成像将成为肝脏和肾脏非侵入性解剖分析的强大新工具。 (C) 2000 Elsevier Science Inc. 保留所有权利。
To establish the usefulness of ESR-CT imaging with 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (carbamoyl-PROXYL) in living animals, we investigated the tissue distribution of carbamoyl-PROXYL after i.v. injection. Ten minutes after injection of carbamoyl-PROXYL, its concentrations in the liver, spleen, kidney, and plasma were higher than those in the small intestine and stomach. However, the inter-organ differences in concentrations were not striking. We selected the liver as a representative organ and attempted to measure the concentration of carbamoyl-PROXYL in it after washing out all of the blood by in situ perfusion with saline. The ESR spectrum of the liver homogenate after complete blood washout revealed that the concentration of carbamoyl-PROXYL was significantly reduced. Thus, at this time, carbamoyl-PROXYL was distributed predominantly in the plasma and/or loosely attached to the surfaces of cells. We obtained high-quality ESR-CT images of the murine abdomen at a measurement time of 40 s and found that a high-intensity area of carbamoyl-PROXYL appeared in the liver and kidneys, indicating an abundant blood circulation. Although the organ specificity of carbamoyl-PROXYL was weak, we consider that ESR-CT imaging with carbamoyl-PROXYL will be a powerful new tool for non-invasive anatomic analysis of the liver and the kidneys. (C) 2000 Elsevier Science Inc. All rights reserved.