Autolysis control and structural changes of purified ficin from Iranian fig latex with synthetic inhibitors.
Autolysis control and structural changes of purified ficin from Iranian fig latex with synthetic inhibitors.
复制标题
从合成抑制剂的伊朗无花果乳胶中纯化纤维蛋白的自溶控制和结构变化。
DOI：
10.1016/j.ijbiomac.2015.12.009
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发表时间：
2016-03
影响因子：
8.2
通讯作者：
Habibi-Rezaei M
中科院分区：
文献类型：
--
作者：
Zare H;Moosavi-Movahedi AA;Salami M;Sheibani N;Khajeh K;Habibi-Rezaei M
无花果蛋白酶是一种半胱氨酸内切蛋白水解酶，在植物的许多生理过程中起着重要作用，在制药、食品等行业有着广泛的应用。在这项工作中，我们报告的抑制和激活的自溶和结构变化与无花果蛋白酶与碘乙酰胺和连四硫酸盐的反应，使用高效液相色谱法（HPLC），超滤膜，动态光散射（DLS）的方法。无花果蛋白酶的结构变化也确定使用紫外吸收，圆二色性（CD），荧光光谱，和差示扫描量热法（DSC）技术。这些技术表明，碘乙酰胺完全抑制无花果蛋白酶自溶，这是不可逆的。连四硫酸盐部分抑制了其自溶，且具有可逆性。无花果蛋白酶的结构变化与两种合成抑制剂与二级结构的变化，减少α-螺旋和增加β折叠和无规卷曲构象，有助于其聚集。
The fig’s ficin is a cysteine endoproteolytic enzyme, which plays fundamental roles in many plant physiological processes, and has many applications in different industries such as pharmaceutical and food. In this work, we report the inhibition and activation of autolysis and structural changes associated with reaction of ficin with iodoacetamide and tetrathionate using high-performance liquid chromatography (HPLC), ultra filtration membrane, and dynamic light scattering (DLS) methods. The ficin structural changes were also determined using UV-absorption, circular dichroism (CD), fluorescence spectroscopy, and differential scanning calorimetry (DSC) techniques. These techniques demonstrated that iodoacetamide completely inhibited ficin autolysis, which was irreversible. However, tetrathionate partially and reversibility inhibited its autolysis. The ficin structural changes with two synthetic inhibitors were associated with secondary structural changes related to decreased alpha-helix and increased beta sheet and random coil conformations, contributing to its aggregation.