Bioglass incorporated methacrylated collagen bioactive ink for 3D printing of bone tissue.
Bioglass incorporated methacrylated collagen bioactive ink for 3D printing of bone tissue.
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DOI：
10.1088/1748-605x/abc744
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发表时间：
2021-02-26
期刊：
影响因子：
--
通讯作者：
Kishore V
中科院分区：
文献类型：
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作者：
Kajave NS;Schmitt T;Nguyen TU;Gaharwar AK;Kishore V
生物活性3D打印支架是骨组织工程（BTE）应用的有前途的候选材料。在这里，我们介绍了一种由生物玻璃45 S5（BG）和甲基丙烯酸化胶原蛋白（CMA）组成的生物活性墨水，用于仿生结构的3D打印，这些仿生结构类似于天然骨组织的有机和无机成分。BG颗粒在胶原网络内的均匀分散改善了胶原水凝胶的稳定性并降低了溶胀。流变学测试显示，在BG掺入后，3D打印构建体的屈服应力和恢复百分比显著改善。此外，添加BG改善了3D打印结构在刺激体液中的骨生物活性。BG掺入CMA（BG-CMA）构建体维持了高细胞活力并增强了人间充质干细胞的碱性磷酸酶活性。此外，与单独的CMA相比，BG-CMA构建体上的细胞介导的钙沉积显著更高。总之，3D打印的BG-CMA结构在BTE应用中具有巨大的潜力。
Bioactive 3D printed scaffolds are promising candidates for bone tissue engineering (BTE) applications. Here, we introduce a bioactive ink composed of Bioglass 45S5 (BG) and methacrylated collagen (CMA) for 3D printing of biomimetic constructs that resemble the organic and inorganic composition of native bone tissue. A uniform dispersion of BG particles within the collagen network improved stability and reduced swelling of collagen hydrogels. Rheological testing showed significant improvement in the yield stress and percent recovery of 3D printed constructs upon BG incorporation. Further, addition of BG improved the bone bioactivity of 3D printed constructs in stimulated body fluid. BG incorporated CMA (BG-CMA) constructs maintained high cell viability and enhanced alkaline phosphatase activity of human mesenchymal stem cells. In addition, cell-mediated calcium deposition was significantly higher on BG-CMA constructs, compared to CMA alone. In conclusion, 3D printed BG-CMA constructs have significant potential for use in BTE applications.