Isoform-dependent immunolocalization of 14-3-3 proteins in developing rat cerebellum
Isoform-dependent immunolocalization of 14-3-3 proteins in developing rat cerebellum
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发育中的大鼠小脑中 14-3-3 蛋白的异构体依赖性免疫定位
DOI：
10.1016/j.brainres.2008.11.065
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发表时间：
2009
期刊：
影响因子：
2.9
通讯作者：
T. Iwamoto
中科院分区：
文献类型：
--
作者：
T. Umahara;T. Uchihara;A. Nakamura;T. Iwamoto
我们利用同工型特异性抗体的免疫印迹和免疫组织化学研究了大鼠小脑出生后发育过程中 14-3-3 蛋白及其 7 种同工型的表达。通过识别其异构体共享序列的抗体 (14-3-3 COM) 探测，总 14-3-3 蛋白的相对量从出生后第 2 天 (P2) 到 P100 没有表现出显着变化。 14-3-3 COM 样免疫反应性 (IR) 最初出现在 P2 处浦肯野细胞的顶端部分，随后扩展到 P14 处的浦肯野细胞体及其树突 (P100)，强度不断增强。分子层（P7 之后）和小脑核神经元（P14 之后）也用该抗体进行免疫标记。这些时间顺序的变化与 β、γ 和 eta 同工型所获得的变化相同。相比之下，类似ε亚型的IR最初在P2的放射状和伯格曼胶质细胞的过程中被识别出来，然后在P7的分子层中出现，随后在P14之后也在浦肯野细胞中增强。 Zeta 和 tau 亚型样 IR 在白质和/或少突胶质细胞中被鉴定。 sigma 同工型是唯一表现出显着数量变化的同工型，峰值位于 P14。 σ亚型的免疫定位最初在 P2 时仅限于浦肯野细胞层的几个细胞中，并在 P14 后转移到外部和内部颗粒细胞以及浦肯野细胞的细胞核，而其免疫标记在 P100 时明显较弱。 7种亚型的不同免疫定位表明，14-3-3蛋白亚型分别与大鼠小脑出生后形成过程中的神经元和胶质细胞增殖、分化、迁移和发育相关。
We investigated the expression of 14-3-3 protein and its 7 isoforms during postnatal development of rat cerebellum with immunoblot and immunohistochemistry with isoform-specific antibodies. The relative amounts of total 14-3-3 protein, probed by an antibody (14-3-3 COM) recognizing a sequence shared among its isoforms, exhibited no significant changes from postnatal day 2 (P2) to P100. 14-3-3 COM-like immunoreactivity (IR), initially in the apical portion of Purkinje cells at P2, extended to Purkinje cell bodies at P14 and to their dendrites (P100) with increasing intensity. Molecular layer (after P7) and cerebellar nucleus neurons (after P14) were also immunolabeled with this antibody. These chronological changes were shared with those obtained with beta, gamma, and eta isoforms. In contrast, epsilon isoform-like IR was initially identified in processes of radial and Bergmann glia at P2 prior to its appearance in the molecular layer at P7 with subsequent intensification also in Purkinje cells after P14. Zeta and tau isoform-like IR was identified in the white matter and/or in oligodendroglial cells. The sigma isoform was the only isoform exhibiting a significant quantitative change with a peak at P14. Immunolocalization of sigma isoform was initially restricted in several cells in Purkinje cell layer at P2 and shifted to nuclei of external and internal granule cells and Purkinje cells after P14, whereas its immunolabeling was markedly weaker at P100. Different immunolocalizations of the 7 isoforms suggest that 14-3-3 protein isoforms individually associate with the neuronal and glial proliferation, differentiation, migration and development during postnatal formation of rat cerebellum.
出生后发育期间小鼠小脑中 IGF-I 下调 14-3-3 eta 基因表达。
DOI：
10.1016/s0165-3806(03)00132-9
发表时间：
2003
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Zhang,Jihui;Popken,GregoryJ;Ye,Ping;D'Ercole,AJoseph
通讯作者：
D'Ercole,AJoseph