Enhancement of cometabolic biodegradation of 4-chlorophenol induced with phenol and glucose as carbon sources by Comamonas testosteroni.
Enhancement of cometabolic biodegradation of 4-chlorophenol induced with phenol and glucose as carbon sources by Comamonas testosteroni.
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DOI：
10.1016/j.jenvman.2010.09.030
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发表时间：
2012-03
影响因子：
8.7
通讯作者：
M. Tobajas;V. Monsalvo;Á. F. Mohedano;J. J. Rodriguez-J.
中科院分区：
文献类型：
--
作者：
M. Tobajas;V. Monsalvo;Á. F. Mohedano;J. J. Rodriguez-J.
研究了睾丸酮丛毛单胞菌CECT 326 T对苯酚和4-氯酚的生物降解。苯酚和4-CP单独作为唯一碳源和能源，但只有苯酚被C.睾丸激素由于共底物的存在可以增强纯培养物对有毒化合物的去除，因此添加苯酚和葡萄糖作为共代谢转化4-CP的生长底物。在两种共底物存在下进行的所有实验中均获得了高效率。尽管事实上，加入葡萄糖减少了4-CP去除的滞后期，但需要较低的苯酚浓度以获得相同的降解效率。4-CP的共代谢转化与苯酚的去除程度密切相关。4-CP/生物量浓度比（S/X）的值用于区分4-CP完全转化（S/X ≤ 0.11）和部分转化（S/X ≥ 0.31），其范围比文献报道的范围窄。在苯酚存在下，共代谢4-CP转化的程度可以通过使用葡萄糖作为额外的碳源和能源来进一步增强。然而，在研究的浓度范围内，没有观察到葡萄糖浓度对4-CP去除的显着影响。
The biological degradation of phenol and 4-chlorophenol (4-CP) by Comamonas testosteroni CECT 326T has been studied. Phenol and 4-CP were treated alone as a sole carbon and energy source, but only phenol was completely degraded by C. testosteroni. Since the presence of cosubstrates can enhance the toxic compounds removal by pure cultures, phenol and glucose were added as growth substrates for cometabolic transformation of 4-CP. High efficiencies were obtained in all the experiments carried out in presence of both cosubstrates. In spite of the fact that the addition of glucose reduced the lag phase of 4-CP removal, lower phenol concentrations were required to obtain the same degradation efficiencies. The cometabolic transformation of 4-CP was closely related with the extent of phenol removal. The values of the 4-CP/biomass concentration ratio (S/X) obtained for discriminating between complete (S/X ≤ 0.11) and partial 4-CP (S/X ≥ 0.31) transformation showed a narrower range than that reported in the literature. The extent of the cometabolic 4-CP transformation in the presence of phenol could be further enhanced by using glucose as an additional carbon and energy source. However, no significant influence of glucose concentration on 4-CP removal was observed over the concentration range studied.