Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition
Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition
复制标题
断奶过渡期间猪胃肠道生态系统微生物多样性研究
DOI：
10.1051/animres:2004019
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发表时间：
2004-07-01
期刊：
影响因子：
--
通讯作者：
Smidt, H
中科院分区：
文献类型：
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作者：
Konstantinov, SR;Favier, CF;Smidt, H
断奶时，受饲粮、环境因素和宿主的影响，仔猪肠道菌群的组成发生了重大的定量和质的变化。在很短的时间内，肠道微生物群最终必须从一个简单、不稳定的群落发展成一个复杂、稳定的群落。在这里，我们提供了基于16S rRNA基因序列多样性的肠道微生物群发育的数据。除了通过克隆和变性梯度凝胶电泳（DGGE）对16S rRNA基因进行pcr分析外，还提供了荧光原位杂交（FISH）数据来量化细菌总群落，主要乳酸菌种群和特定乳酸菌种。本文报道的结果表明，添加不可消化的、可发酵的碳水化合物（=益生元）会导致小肠中乳酸杆菌的富集，并增加结肠中细菌群落的稳定性和多样性。这些数据支持了饮食改变可以调节肠道微生物群组成的假设。这些发现可能对旨在改善断奶过程中动物健康的饮食策略的发展具有潜在的重大意义。
At the time of weaning, major quantitative and qualitative changes occur in the composition of the intestinal microbiota of piglets, influenced by diet, environmental factors, and the host. Within a short period of time, the intestinal microbiota must ultimately develop from a simple, unstable community into a complex and stable one. Here we present data on the development of the intestinal microbiota based on 16S rRNA gene sequence diversity. In addition to a PCR-based analysis of the 16S rRNA gene by cloning and denaturing gradient gel electrophoresis (DGGE), data on fluorescent in situ hybridisation (FISH) are presented to quantify the total bacterial communities, major Lactobacillus populations and specific Lactobacillus species. The results reported here indicate that the addition of non-digestible, fermentable carbohydrates (= prebiotics) leads to an enrichment of lactobacilli in the small intestine, and increased stability and diversity of the bacterial community in the colon. The data support the hypothesis that changes of the diet can modulate the composition of the microbiota in the intestine. These findings may have potentially major implications for the development of dietary strategies aiming to improve animal health during the weaning process.