Biologically active precursor for transforming growth factor type alpha, released by retrovirally transformed cells.
Biologically active precursor for transforming growth factor type alpha, released by retrovirally transformed cells.
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转化生长因子α型的生物活性前体，由逆转录病毒转化细胞释放。
DOI：
10.1073/pnas.83.17.6307
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发表时间：
1986
影响因子：
11.1
通讯作者：
Massagué,J
中科院分区：
文献类型：
--
作者：
Ignotz,RA;Kelly,B;Davis,RJ;Massagué,J
逆转录病毒转化的大鼠细胞积极表达转化生长因子-α(TGF-α)，将一种17-19 kDa的可溶性蛋白释放到培养液中，该蛋白已被确认为转化生长因子-α的前体。根据特定抗体的识别和弹性蛋白酶将其转化为具有成熟的转化生长因子-α性质的6-kDa多肽的能力，确定该蛋白为转化生长因子-α前体。这种转化生长因子-α前体与表皮生长因子/转化生长因子-α受体结合，激活完整细胞中与受体相关的酪氨酸激酶活性。该前体在体外转化为成熟的转化生长因子-α后，其生物学效力并未显著增强。这些研究表明，转化的细胞能够释放一种能够在体内产生强烈有丝分裂作用的转化生长因子-α前体。
Retrovirus-transformed rat cells that actively express transforming growth factor type alpha (TGF-alpha) release into the medium a soluble protein of 17-19 kDa that has been identified as a precursor for TGF-alpha. The identification of this protein as a TGF-alpha precursor is based on its recognition by specific antibodies and by the ability of elastase to convert this protein into a 6-kDa polypeptide with the properties of mature TGF-alpha. This TGF-alpha precursor binds to epidermal growth factor/TGF-alpha receptors and activates the receptor-associated tyrosine kinase activity in intact cells. The biological potency of this precursor is not markedly increased by conversion into mature TGF-alpha in vitro. These studies demonstrate the ability of transformed cells to release a TGF-alpha precursor capable of strong mitogenic action in vivo.