A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues
A simple microindentation technique for mapping the microscale compliance of soft hydrated materials and tissues
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DOI：
10.1002/jbm.a.30812
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发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Wong, Joyce Y.
中科院分区：
文献类型：
--
作者：
Jacot, Jeffrey G.;Dianis, Scott;Wong, Joyce Y.
最近的几项研究表明，细胞响应于软基质上的弹性模量和弹性梯度。然而，用于测量弹性模量的传统宏观尺度方法不能解决弹性梯度或分层组织的宏观尺度和微观尺度弹性模量之间的差异。在这里，我们提出了一种技术，用于测量的软，水合凝胶和组织的微尺度弹性模量。这种技术需要比同等的原子力显微镜（AFM）更少的设备，可以很容易地测量更大的样品与高分辨率。我们通过测量具有不依赖于测量尺度的弹性的水凝胶的微尺度模量来验证这种技术。我们表明，使用显微压痕测量的弹性模量与使用AFM和宏观尺度的拉伸模量的测量。我们验证了该技术的能力，以表征水凝胶的弹性梯度为2.2 kPa/mm，横跨19 mm，并测量的微尺度的弹性模量的内皮侧的人大隐静脉，这是一个数量级小于整个静脉的宏观尺度模量。这种简单，廉价的系统允许测量的空间组织的微尺度弹性性能的完全水合，软凝胶和组织作为一个常规的实验室技术。(c)2006 Wiley Periodicals，Inc.
Several recent studies have shown that cells respond to the elastic modulus and elasticity gradients on soft substrates. However, traditional macroscale methods for measuring elastic modulus cannot resolve elastic gradients or differences between the macroscale and microscale elastic modulus of layered tissues. Here, we present a technique for measurement of the microscale elastic modulus of soft, hydrated gels and tissues. This technique requires less equipment than equivalent atomic force microscopy (AFM) and can easily measure larger samples with high adhesiveness. We validate this technique by measuring the microscale modulus of a hydrogel with elasticity that does not depend on measurement scale. We show that the elastic modulus measured using microindentation correlates with measurements using AFM and the macroscale tensile modulus. We verified the ability of this technique to characterize a hydrogel with an elastic gradient of 2.2 kPa/mm across 19 mm and to measure the microscale elastic modulus of the endothelial side of human greater saphenous vein, which is an order of magnitude less than the whole vein macroscale modulus. This simple, inexpensive system allows the measurement of the spatial organization of microscale elastic properties of fully hydrated, soft gels and tissues as a routine laboratory technique. (c) 2006 Wiley Periodicals, Inc.