STIMULATION OF EPITHELIAL-CELL GROWTH BY THE NEUROPEPTIDE SUBSTANCE-P
STIMULATION OF EPITHELIAL-CELL GROWTH BY THE NEUROPEPTIDE SUBSTANCE-P
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DOI：
10.1002/jcb.240520411
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发表时间：
1993-08-01
影响因子：
4
通讯作者：
MANNIS, MJ
中科院分区：
文献类型：
--
作者：
REID, TW;MURPHY, CJ;MANNIS, MJ
发现神经肽物质P（SP）在无血清环境中刺激上皮细胞（角膜和透镜）的DNA合成和细胞生长。治疗时间的长度被证明是重要的，因为更长的时间使剂量-反应曲线向左移动。在短期DNA合成研究（40小时）中，SP的刺激（或与胰岛素的协同作用）直到接近10 μ M时才明显，然而，当DNA合成测定进行很长一段时间（5天）时，观察到SP的刺激1 pM。SP对透镜上皮细胞DNA合成的刺激作用与胰岛素有协同作用，但对角膜上皮细胞的刺激作用不明显。对透镜上皮细胞SP的细胞生长研究也显示其自身的生长刺激作用和与1-2 pM浓度的胰岛素的协同作用。神经肽降钙素基因相关肽（CGRP）在高达2.5 μ M的浓度下对上皮细胞本身没有显示出DNA合成刺激能力;然而，在0.025 μ M的浓度下，它与SP具有协同作用。SP预处理上皮细胞2小时，导致细胞对随后加入SP或胰岛素的敏感性增加。这种增加与以下假设一致：SP的信号在其从细胞中去除后仍然存在，或者SP从其受体的解离时间长于洗涤时间。(C)1993 Wiley-Liss，Inc.
The neuropeptide substance P (SP) was found to stimulate DNA synthesis and cell growth for epithelial cells (cornea and lens) in a serum-free environment. The length of treatment time was shown to be important since longer times shifted the dose-response curve to the left. In short-term DNA synthesis studies (40 h) the stimulation with SP (or synergism with insulin) was not apparent until close to 10 muM, however, when DNA synthesis assays were carried out over a long period of time (5 days) stimulation with SP was seen at 1 pM. The stimulation of DNA synthesis by SP was synergistic with insulin for lens epithelial cells, but little synergism was seen with corneal epithelial cells. It cell growth studies on lens epithelial cells SP also showed growth stimulation by itself and synergism with insulin at concentrations of 1-2 pM. The neuropeptide calcitonin gene related peptide (CGRP) showed no DNA synthesis stimulating ability on epithelial cells by itself at concentrations as high as 2.5 muM; however, it was synergistic with SP at a concentration of 0.025 muM. SP pretreatment of epithelial cells for 2 h causes an increase in cellular sensitivity to subsequent addition of either SP or insulin. This increase is consistent with the hypothesis that either the signal from SP persists after its removal from the cell or the dissociation time for SP from its receptor is longer than the wash time. (C) 1993 Wiley-Liss, Inc.