Aggregatibacter actinomycetemcomitans serotypes and DGGE subtypes in Thai adults with chronic periodontitis
Aggregatibacter actinomycetemcomitans serotypes and DGGE subtypes in Thai adults with chronic periodontitis
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DOI：
10.1016/j.archoralbio.2015.09.003
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发表时间：
2015-12-01
影响因子：
3
通讯作者：
Teanpaisan, Rawee
中科院分区：
文献类型：
--
作者：
Pahumunto, Nuntiya;Ruangsri, Praphansri;Teanpaisan, Rawee
目的：了解泰国慢性牙周炎患者分离株放线菌聚集菌血清型及DGGE亚型的分布。设计：通过全口记录评估44例泰国成人牙周炎患者的CAL PPD和BOP。在选择性TSBV琼脂上，从深袋中分离到放线菌孢子菌79株，从浅袋中分离到17株。用PCR分型，用DGGE分型。结果：放线菌孢子虫患病率为84.1%。无血清分型的放线菌球菌与血清分型的发生率相当（54.5%）；血清a型18.2%，血清c型15.9%，血清e型9.1%，血清f 11.4%。血清b型和d型未检出。从两例患者中分离到一株类似JP2但血清型为c的菌株，另外两株放线菌comitans分离株的ltx启动子上有886 bp的插入。DGGE分型在非血清分型菌株中发现16种不同的亚型。其中2个亚型（NS1和NS2）的发生率分别为12.7%和10.1%，高于其他14个亚型(
Objective: To investigate the distribution of Aggregatibacter actinomycetemcomitans serotypes and DGGE subtypes among isolates from Thai chronic periodontitis patients.Design: Forty-four adult Thai periodontitis patients were assessed by a full mouth recording for CAL PPD, and BOP. Seventy-nine strains of A. actinomycetemcomitans were isolated from deep pockets on selective TSBV agar and 17 strains were isolated from shallow pockets. The strains were serotyped using PCR and subtyped using DGGE.Results: The prevalence of A. actinomycetemcomitans was 84.1%. Non-serotypeable A. actinomycetemcomitans strains occurred equally frequent as serotypeable (54.5%); serotype a 18.2%, serotype c 15.9%, serotype e 9.1%, and serotype f 11.4%. Serotype b and d were not detected. A JP2 like strain but serotyped as c was isolated from two patients, and another two strains showed an 886 bp insertion on the ltx promoter of their A. actinomycetemcomitans isolates. DGGE typing disclosed 16 different subtypes among the non-serotypeable strains. Two of them (NS1 and NS2) were more common (12.7 and 10.1%) among the strains than the other 14 subtypes (