Binding of Dissolved Organic Matter to RNA and Protection from Nuclease-Mediated Degradation
Binding of Dissolved Organic Matter to RNA and Protection from Nuclease-Mediated Degradation
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溶解的有机物与 RNA 的结合以及防止核酸酶介导的降解
DOI：
10.1021/acs.est.3c05019
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发表时间：
2023
影响因子：
11.4
通讯作者：
Parker, Kimberly M.
中科院分区：
文献类型：
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作者：
Chatterjee, Anamika;Zhang, Ke;Parker, Kimberly M.
RNA在环境系统中的持久性是新兴应用的一个重要参数，包括生态调查、基于废水的流行病学和RNA干扰生物农药。RNA的持久性受其生物降解速率的控制，特别是胞外酶，尽管决定这一速率的具体因素尚未得到表征。由于以前的工作表明，核酸，特别是DNA与溶解的有机物（DOM）相互作用，我们假设DOM可能会结合RNA，并阻碍其在自然系统中的生物降解。我们首先采用了以前用于评估RNA-蛋白质结合的技术，以区分在所有位点被DOM结合的RNA与未被DOM结合或部分被DOM结合的RNA。这项技术的结果表明，腐殖酸结合RNA更广泛的比富里酸。在8-10 mgC/L的浓度下，还发现腐殖酸比富里酸更有效地抑制RNA的酶降解。在含有DOM的地表水和土壤提取物中，相对于pH调节的对照，RNA降解被抑制了39-46%。由于DOM结合和抑制RNA酶降解的能力，在高DOM浓度的环境系统中，RNA生物降解可能会减慢，这可能会增加其持久性。
The persistence of RNA in environmental systems is an important parameter for emerging applications, including ecological surveys, wastewater-based epidemiology, and RNA interference biopesticides. RNA persistence is controlled by its rate of biodegradation, particularly by extracellular enzymes, although the specific factors determining this rate have not been characterized. Due to prior work suggesting that nucleic acids–specifically DNA–interact with dissolved organic matter (DOM), we hypothesized that DOM may bind RNA and impede its biodegradation in natural systems. We first adapted a technique previously used to assess RNA-protein binding to differentiate RNA that is bound at all sites by DOM from RNA that is unbound or partially bound by DOM. Results from this technique suggested that humic acids bound RNA more extensively than fulvic acids. At concentrations of 8–10 mgC/L, humic acids were also found to be more effective than fulvic acids at suppressing enzymatic degradation of RNA. In surface water and soil extract containing DOM, RNA degradation was suppressed by 39–46% relative to pH-adjusted controls. Due to the ability of DOM to both bind and suppress the enzymatic degradation of RNA, RNA biodegradation may be slowed in environmental systems with high DOM concentrations, which may increase its persistence.