Reticulate evolution in the apogamous Dryopteris varia complex (Dryopteridaceae, subg. Erythrovariae, sect. Variae) and its related sexual species in Japan
Reticulate evolution in the apogamous Dryopteris varia complex (Dryopteridaceae, subg. Erythrovariae, sect. Variae) and its related sexual species in Japan
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DOI：
10.1007/s10265-014-0652-0
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发表时间：
2014-11-01
影响因子：
2.8
通讯作者：
Murakami, Noriaki
中科院分区：
文献类型：
--
作者：
Hori, Kiyotaka;Tono, Akitaka;Murakami, Noriaki
无配生殖的蕨类植物由于其连续的形态变异，常常很难与近缘种区分开来。为了阐明变异鳞毛蕨复合体成员之间的遗传关系，我们分析了质体基因rbcL和核基因PgiC的核苷酸序列。对二倍体有性种D. caudipinna和D. chinensis，但最近的分子系统学研究表明它们与该复合体关系密切。二倍体有性种D. varia、异枝藓D.虎耳草D. sp. 'protobissetiana'（未描述的二倍体有性种），D. caudipinna，和D. chinensis，分别命名为A、B、C、D和E。因此，该复合体中无配生殖种的PgiC组成如下：D. bissetiana，B + C; D. kobayashii，B + C + E）; pacifica、A + C、A + B + C或A + C + D; sacrosancta，A + C + E; D.虎耳草属（saxifragivaria），B + C.这些结果表明，这些无配生殖种不仅是由D.变异复合体（A，B，和C），但也有两个二倍体有性种D。caudipinna（D）和尾翅杜父鱼D. chinensis（E），不属于复合体。
Apogamous fern species are often difficult to distinguish from related species because of their continuous morphological variations. To clarify the genetic relationships among the members of the Dryopteris varia complex, we analyzed the nucleotide sequences of the plastid gene rbcL and the nuclear gene PgiC. We also analyzed the diploid sexual species D. caudipinna and D. chinensis, which have not been included in the complex, but were recently shown to be closely related to the complex in a molecular phylogenetic study. The PgiC sequences of the diploid sexual species, D. varia, D. saxifraga, D. sp. 'protobissetiana' (undescribed diploid sexual species), D. caudipinna, and D. chinensis, were well differentiated and hence designated A, B, C, D, and E, respectively. Thus, the PgiC constitution of apogamous species in the complex was as follows: D. bissetiana, B + C; D. kobayashii, B + C + E); D. pacifica, A + C, A + B + C, or A + C + D; D. sacrosancta, A + C + E; and D. saxifragivaria, B + C. These results suggest that these apogamous species are formed by hybridizations of species including not only the three diploid sexual species of the D. varia complex (A, B, and C) but also the two diploid sexual species D. caudipinna (D) and D. chinensis (E), which do not belong to the complex.